Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells
- Jinglei Cai1,
- Yanmei Zhang1,
- Pengfei Liu1, 2,
- Shubin Chen1,
- Xuan Wu1, 2,
- Yuhua Sun3,
- Ang Li4,
- Ke Huang1,
- Rongping Luo1,
- Lihui Wang1,
- Ying Liu1, 5,
- Ting Zhou1,
- Shicheng Wei3, 6,
- Guangjin Pan1 and
- Duanqing Pei1Email author
© Cai et al.; licensee BioMed Central Ltd. 2013
Received: 9 May 2013
Accepted: 8 June 2013
Published: 30 July 2013
Tooth is vital not only for a good smile, but also good health. Yet, we lose tooth regularly due to accidents or diseases. An ideal solution to this problem is to regenerate tooth with patients’ own cells. Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells (ifhU-iPSCs).
We first differentiated ifhU-iPSCs to epithelial sheets, which were then recombined with E14.5 mouse dental mesenchymes. Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30% for 8 different iPSC lines, comparable to H1 hESC. We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures, possessing physical properties such as elastic modulus and hardness found in the regular human tooth.
Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies.
KeywordsHuman urine Integration-free iPSCs Recombinant tooth Bioengineered tooth Dental epithelium
integration-free human urine induced pluripotent stem cells
Embryonic day 14.5
human embryonic stem cell
Dental pulp stem cells
Periodontal ligament stem cells
Gum stem cells
induced pluripotent stem cells
human urine cells
Bone morphogenetic protein 4
Defined keratinocyte serum-free medium and supplement
Scanning electronic microscope
Transmission electron microscopy
Human leukocyte antigen-I
human nucleus antigen
Dulbecco’s modified eagle’s medium
Fetal bovine serum
The goal of regenerative medicine is to regenerate fully functional tissues or organs that can replace lost or damaged ones occurred during diseases, injury and aging [1, 2]. The advent of iPSCs should speed up the application of regenerative tissues or organs such as tooth in the clinic . While iPSC-derived cells have been tested in animal models [4–7], no solid organs or tissues such as tooth have been generated with human iPSCs. The tooth represents one of the best experimental models in organogenesis [8, 9], and is easily accessible for human replacement therapy . Tooth is formed by reciprocal interactions between epithelium and mesenchymal cells derived from the cranial neural crest [11, 12]. Developmentally, the odontogenic potential shifts from the dental epithelium to dental mesenchyme at bud stage (Embryonic day 12, E12) [13, 14]. Then, the epithelium differentiates into ameloblasts and finally forms the enamel, while the mesenchyme differentiates into the dentin, cementum and dental pulp . Tooth stem cells such as dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and gum stem cells (GSCs) have been isolated and investigated for tooth regeneration [16–18]. Recently, Arakaki and colleagues reported that mouse iPSCs (miPSCs) could be differentiated into ameloblasts via interactions with dental epithelium and these miPSCs derived epithelial cells were positive with the epithelial cell markers p63, cytokeratin-14 (K14), and ameloblast markers ameloblastin and enamelin . Shortly after, another group differentiated miPSCs into neural crest-like cells to generate odontoblasts that express dental mesenchyme markers Msx1, Pax9, Lhx6, and odontoblast marker dentin sialoprotein (DSP) . Recently, miPSCs were mixed with dissociated mouse dental epithelial and mesenchymal cells and found to contribute to odontogenesis in vivo . However, human iPSCs have not yet been explored for tooth regeneration.
We developed a chimeric culture system for tooth regeneration from human iPSCs (Additional file 1). We have a collection of both H1 hESCs as a control and iPSCs derived in our laboratories, mostly from human urine cells (hU) by oriP/EBNA episomal vectors carrying a combination of reprogramming factors Oct4, Sox2, SV40LT, Klf4 and miR302/367 through electroporation [22, 23]. We hypothesized that hESCs or hiPSCs can be induced to produce epithelial sheets capable of replacing the E14.5 mouse dental epithelium in a reconstitution process (Additional file 1). The reconstituted explants can then be cultured in vitro for 1–2 days and transplanted beneath mouse subrenal capsule for 3 weeks for tooth regeneration (Additional file 1).
Epithelial sheets generated from hESCs and iPSCs
Tooth-like structures generated from hESC and iPSC derived epithelial sheets
We then analyzed the hardness and elastic modulus of human adult teeth (human group), 3-week mouse teeth developed from tooth germs under kidney capsule (Mouse WT group), and the regenerative teeth from H1-ESCs and ifhU-iPSCs groups by Nano-indentation (Figure 2D). The harness and elastic modulus of dentin and enamel in above five groups showed similar properties (Figure 2D). In the case of enamel, the hardness and elastic modulus of H1-ESCs and two ifhU-iPSCs groups were lower than those of human and Mouse WT groups (Figure 2D, right graphs). Interestingly, the hardness of ifhU5-iPSCs group was around 4 times higher than that of H1-ESCs group (Figure 2D, right graphs). Impressively, the hardness of ifhU5-iPSCs group reached 1/3 by comparison with that of human group (Figure 2D, right graphs). We further analyzed the chemical composition of the teeth by Raman spectroscopy. We showed that they all have similar spectra with comparable intensity (Figure 2E). In the five groups examined, the enamel of the teeth are highly mineralized and the spectra showed almost the same spectrum as hydroxyapatite [Ca5(PO4)3OH, the primary mineral in teeth] (data not shown). These tooth-like structures also showed similar signs of protein with Raman peaks in dentin, including the hydroxyapatite peak compared to human teeth (Figure 2E). These results indicated that the regenerative teeth from both H1-ESCs and two ifhU-iPSCs groups have similar constituents with human and mouse teeth. We analyzed a total of 8 hiPSC lines from three urine donors (U1, 5, 7) for tooth regeneration and had a success rate of up to 30% (Figure 2F).
In this study, we demonstrate that epithelial sheets derived from ifhU-iPSCs can functionally substitute tooth germ epithelium to regenerate tooth-like structures when combined with mouse dental mesenchyme in vivo. Recently, two reports have showed that mouse iPSCs could be induced to differentiate into amelobastin-expressing dental epithelial cells and odontogenic mesenchymal cells through neural crest-like cells respectively [19, 20]. In contrast, our approach provides an ideal model for the regeneration of patient specific tooth in the future.
It is well known that adult dental stem cells have been successfully applied in tissue engineering research. It has proven to be possible to generate dentin pulp complexes, even whole teeth out of isolated cells . However, some required and major issues need to be solved before these developing approaches being translated to the dental clinic. Probably the most important limiting factor is the absence of consistent sources of epithelial stem cells with odontogenic potential in the adult human individual. Periodontal ligament cells and postnatal oral mucosal epithelial cells are considered as the possible epithelial substitutes. Recently, human newborn skin keratinocyte progenitors were used to form tooth structures by recombination. However, FGF proteins are required for the formation of ameloblasts . More recently, adult human gingival epithelial cells were reported to give rise to ameloblast-like cells in an bioengineered tooth when combined with mouse dental mesenchyme . Although these approaches could lead to tooth formation, the sources of endogenous dental epithelial stem cells seem to be scarce. Thus, an appealing alternative would be to obtain the epithelial substitutes from autogenic iPSCs. Here, we succeeded in inducing intergration-free human urine iPSCs into the intact epithelial sheet, which developed into ameloblasts in a tooth-like structure. This positive experience indicates that human iPSCs could be applied on tooth regeneration.
Although our approach displayed iPSC contribution only to the epithelial substitutes, a dental engineering process generated from human cells could be easily designed. As the ectomesenchymal stem cells, such as human dental pulp stem cells can generate a complete dentin-pulp complex and peridontium. The hard enamel tissue constituting the remaining part of the tooth could be formed by human iPSC derived epithelial sheet described here. Therefore, success of our approach confirmed that autogenic iPSCs could be used as a source of new dental epithelium to be recombined with ectomesenchymal cells, thus creating a bioengineered tooth germ that can be cultured in vitro and transplanted to the jawbone of a recipient host to form a fully function tooth . Of course, it remains more work for generating our iPSC derived epithelial sheet with odontogenic potential based on detailed analysis of molecular events associated epithelial-mesenchymal interactions, a theme recurrent throughout development [28–30]. On the other hand, future work should focus on the derivation of odontogenic cells and/or dental mesenchyme from ifhU-iPSCs which will be useful for the final dream of total regeneration of human tooth for clinical therapy.
In conclusion, human iPSCs are capable of contribution on tooth generation by pre-differentiating into epithelial sheets and further responding to odontogenic signals from embryonic dental mesenchyme. In particular, these regenerative teeth contain enamel with ameloblast-like cells of human origin and possess physical properties found in the regular human tooth. Thus, human iPSCs could be a candidate source of seed cells on human tooth tissue-engineering for further drug screening or regenerative therapies.
Cell culture and epithelial differentiation
We used 9 distinct lines of pluripotent cells in this study. These included hESCs (WA01/H1; WiCell, USA). 8 hiPSC lines derived from urine cells of three donors (U1, 5, 7) were obtained from South Stem Cell Bank in China. Epithelial differentiation of hESCs and iPSCs was carried out with differentiation medium (DMEM/F12 [Gibco] supplemented with 1× N2 supplement, 1 μM RA and 25 ng/ml BMP4) when hESCs or iPSCs reach 70-80% confluence. Medium was replaced directly at D7 with defined keratinocyte serum-free medium and supplement (DSFM) to the confluent cell layer. In addition, differentiated cells at D7 could be detached with Dispase (BD, Cat. NO. 17105–041) and distributed on gelatin-coated plates at a split ratio of 1:3 with culture medium (DSFM) changing every other day till D42. Detailed methods and any associated references are available in an Additional file 2.
Reconstitution of bioengineered tooth germs and subrenal capsule assays
The molar tooth germs were dissected from the mandibles of E14.5 mice and incubated in 0.75 mg/ml Dispase (BD) for 40 min at 37°C. The dental mesenchyme was separated from the dental epithelium. Meanwhile, hESCs or iPSCs derived epithelial cells at D7 were harvested as an intact epithelial sheet using 2 mg/ml Dispase (BD). A piece of dissected epithelial sheet was placed on the top of one mouse dental mesenchyme. The recombinant explants were cultured with Trowell-type system in DMEM containing 10% FBS for 1–2 days before being transplanted into the renal subcapsular layer of adult nude mice. Usually, 6–8 recombinant specimens were transplanted into per kidney. After 3 weeks, the host mice were sacrificed, and kidneys were dissected to obtain the calcified tissues.
The calcified tissues were decalcified and sectioned in a 5 μm thickness. For immunohistochemistry, the primary antibodies used were anti-Ameloblastin (Amel; 1:100, Santa), anti-human leukocyte antigen I (HLA-I, 1:50, abcam) and anti-bone sialoprotein (BSP; 1:100, Millipore) polyclonal antibodies. For immunofluorescence, the primary antibodies used were anti-human nucleus antigen (hNA, 1:500, Millipore), anti-Cytokeratin 14 (K14; 1:100, Santa) and anti-p63 (p63; 1:100, Invitrogen) polyclonal antibodies.
Real-time quantitative PCR
Total RNA was extracted with Trizol (Invitrogen). 2 μg of RNA was reverse transcribed using RT-PCR kit (Takara) and qPCR was performed using a Thermal Cycler Dice7™ Real Time System and SYBR Green Premix EX Taq™ (Takara).
The primary antibodies used were anti-Oct4 (Oct4 pAb; 1:1000), anti-Cytokeratin 18 (K18 pAb; 1:1000) and anti-p63 (p63 pAb; 1:1000) polyclonal antibodies. Anti-mouse or goat HRP was used to detect protein together with the Amersham ECL kit (GE Healthcare).
Scanning electronic microscope (SEM) and transmission electron microscopy (TEM)
The differentiating cells at D7, D14 were prefixed with 2.5% glutaraldehyde for 24 h at 4°C, and postfixed with 1% osmium tetroxide for 2 h. For SEM, the samples were examined by scanning electron microscopy (PHILIPS, XL-30ESEM). For TEM, the samples were examined with transmission electron microscope (PHILIPS, TECNAI-10).
The teeth profiles were exposure after embedded with epoxy resins and opened packet buy piece for further examination. Nano indentation hardness calculation formula is: H = P max/A (h c). [H: hardness; P max: the largest indentation force; A (h c): projection area of the indentation].
Raman spectroscopy assay
Spontaneous Raman spectra were acquired with a confocal Raman spectrometer (Labram HR800, Horiba JobinYvon) under 784.4 nm excitation laser through a 50x objective. The integration time was 10 s. The spectrometer was calibrated with the Raman line of silicon at 520.7 cm-1.
We thank Prof. Yanding Zhang and Prof. Dajiang Qin for valuable suggestions and all staffs working for the South Stem Cell Bank. This work was supported by the grants from Ministry of Science and Technology 973 Program (2010CB944800, 2011CB965200), National Natural Science Foundation of China (31000402), the “Strategic Priority Research Program” of the Chinese Academy of Sciences (XDA01020401, XDA01020202), 863 Program (2011AA020109), Ministry of Science and Technology International Technology Cooperation Program (2012DFH30050) and Open Project of Key Laboratory of Regenerative Biology, Chinese Academy of Sciences (KLRB201217).
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