From: Defining synthetic surfaces for human pluripotent stem cell culture
Substrates | Ligands or ECMPs | CAM(s) | References |
---|---|---|---|
Tissue culture polystyrene (TCP) | Vitronectin | αVβ3/5 integrins, GAGs | [7] |
Laminin-511 | α6β1 integrin | [11] | |
Laminin E8 fragments | - | [13] | |
Fibronectin | α5β1 integrin | [48] | |
Collagen + fibronectin + laminin + vitronectin | - | [125] | |
Poly(L-lysine) | - | [126] | |
E-cadherin-IgG1Fc (StemAdhereTM) | E-cadherin | [10] | |
Amine-modified TCP | Cyclic-CRGDC | - | [86] |
UV-treated TCP | Adsorbed serum proteins, vitronectin | - | [12] |
Acrylate monomer-coated TCP | KGGNGEPRGDTYRAY | αVβ5 | [9] |
(Corning SynthemaxTM) | integrins - αVβ3/5 | [9] | |
KGGPQVTRGDVFTMP | |||
Vitronectin | integrins, GAGs | [42] | |
Self-assembled monolayers | GKKQRFRHRNRKG | HSPGs | [79] |
LTTAPKLPKVTR | GAGs | [127] | |
Amino-propylmethacrylamide | BSA + non-specific proteins (adsorbed from media) | - | [14] |
Hydrogels | |||
Polyacrylamide hydrogel | GKKQRFRHRNRKG | HSPGs | [92] |
PMEDSAH | Unknown. Adsorbed growth factors? | - | [99] |