GATA2−/− human ESCs undergo attenuated endothelial to hematopoietic transition and thereafter granulocyte commitment
- Ke Huang1, 2,
- Juan Du1, 2,
- Ning Ma1, 2,
- Jiajun Liu3,
- Pengfei Wu1, 2,
- Xiaoya Dong3,
- Minghui Meng1, 2,
- Wenqian Wang3,
- Xin Chen1, 2,
- Xi Shi1, 2,
- Qianyu Chen1, 2,
- Zhongzhou Yang1, 2,
- Shubin Chen1, 2,
- Jian Zhang1, 2,
- Yuhang Li4,
- Wei Li1, 2,
- Yi Zheng1, 2,
- Jinglei Cai1, 2,
- Peng Li1, 2,
- Xiaofang Sun5, 6,
- Jinyong Wang1, 2,
- Duanqing Pei1, 2 and
- Guangjin Pan1, 2Email author
© Huang et al. 2015
Received: 20 May 2015
Accepted: 8 July 2015
Published: 5 August 2015
Hematopoiesis is a progressive process collectively controlled by an elaborate network of transcription factors (TFs). Among these TFs, GATA2 has been implicated to be critical for regulating multiple steps of hematopoiesis in mouse models. However, whether similar function of GATA2 is conserved in human hematopoiesis, especially during early embryonic development stage, is largely unknown.
To examine the role of GATA2 in human background, we generated homozygous GATA2 knockout human embryonic stem cells (GATA2 −/− hESCs) and analyzed their blood differentiation potential. Our results demonstrated that GATA2 −/− hESCs displayed attenuated generation of CD34+CD43+ hematopoietic progenitor cells (HPCs), due to the impairment of endothelial to hematopoietic transition (EHT). Interestingly, GATA2 −/− hESCs retained the potential to generate erythroblasts and macrophages, but never granulocytes. We further identified that SPI1 downregulation was partially responsible for the defects of GATA2 −/− hESCs in generation of CD34+CD43+ HPCs and granulocytes. Furthermore, we found that GATA2 −/− hESCs restored the granulocyte potential in the presence of Notch signaling.
Our findings revealed the essential roles of GATA2 in EHT and granulocyte development through regulating SPI1, and uncovered a role of Notch signaling in granulocyte generation during hematopoiesis modeled by human ESCs.
KeywordshESCs GATA2 EHT HPC Granulocyte Notch signaling
Hematopoiesis is a complex process that involves multiple developmental processes, such as cellular proliferation, differentiation, and survival. This process is accurately controlled by the coordination of a set of transcription factors and diverse signaling pathways [1–5]. GATA2 belongs to the transcriptional regulatory GATA protein family and is broadly expressed in hematopoietic cells, particularly in hematopoietic progenitors [6, 7]. The essential function of GATA2 in genesis, differentiation, and even trans-differentiation of hematopoietic stem/or progenitor cells (HSCs or HPCs) has been extensively examined [8, 9]. A GATA2-deficient mouse exhibited severe anemia and died at early stage of gestation due to a reduced number of primitive erythroid cells and HPCs , highlighting the essential role of GATA2 in early hematopoiesis. Furthermore, GATA2 is also crucial in maintaining the proliferation and normal function of adult HSCs or HPCs [7, 11–13]. Recently, de Pater et al. demonstrated that GATA2-deficient hemogenic endothelium (HE) failed to generate long-term repopulating HSCs due to the impairment of endothelial to hematopoietic transition (EHT) . Mechanistically, GATA2 might regulate HPCs through direct activation of other critical factors. For instance, Pimanda et al. described that Gata2, Fli1, and Scl/Tal1 formed a regulatory circuit to regulate early hematopoietic development in the mouse model .
Besides HSCs or HPCs, GATA2 also regulates hematopoietic lineage specification. For example, overexpression of GATA2 in primary erythroid progenitor cells promoted megakaryocyte differentiation while inhibiting erythrocyte differentiation . Adult bone marrow from GATA2 heterozygous mice (GATA2 +/−) exhibited reduced function of granulocyte-macrophage progenitors (GMPs) . The diverse roles of GATA2 in different hematopoietic lineages indicate that its function is largely cell context-dependent [17, 18]. Given that most of the data available now on how GATA2 regulates hematopoiesis are obtained from the murine system, its roles in human background remain elusive and require further investigation.
Human embryonic stem cells (hESCs) are capable of hematopoietic differentiation in vitro and thus could serve as a valuable model for investigating early human hematopoiesis. They could efficiently differentiate into HPCs as well as different hematopoietic lineages, through either co-culturing with stromal cells or embryoid body (EB) formation in the presence of specific cytokines [19, 20]. The hESC-derived HPCs exhibited typical phenotype of blood progenitors, including expressing surface markers as well as forming different blood lineage colonies (colony-forming cells, CFCs). Moreover, most studies to date support that the in vitro blood differentiation of hESCs was a controlled sequential process starting from the early embryonic mesoderm, via HE and HPCs to mature blood cells, recapitulating hematopoietic development in vivo [21, 22]. Therefore, it could serve as a good system to examine the role of GATA2 during early human hematopoiesis.
In this report, through gene targeting, we generated GATA2 −/− human ESCs and analyzed their hematopoietic differentiation potential. Through examining surface markers that were previously identified in hESC-derived HPCs (CD34+CD43+) [23, 24], we found that GATA2 −/− hESCs generated much less HPCs both in the OP9 co-culturing system and a stromal-free medium that could drive blood differentiation. However, GATA2 −/− hESCs retained the potential to produce the major subtype blood lineages, such as erythroblasts and macrophages. In contrast, we observed a complete defect of GATA2 −/− hESCs in generating granulocytes in OP9-driven blood differentiation. Mechanistically, we identified that the granulocyte defect was partially due to the downregulation of SPI1, a critical transcription factor known for myeloid and lymphocyte development in the mouse model. Enforced expression of SPI1 rescued the production of granulocytes of GATA2 −/− hESCs in co-culturing with OP9. Interestingly, GATA2 −/− hESCs restored the potential when co-culturing with OP9 expressing DL1, the Notch signaling ligand. Thus, our findings revealed the critical roles of GATA2 in EHT and granulocyte development in human-modeled hematopoiesis.
Generation of GATA2 −/− human ESCs
GATA2 −/− hESCs generate reduced HPCs due to EHT defect
To further analyze the function of HEs from GATA2 −/− hESCs, we sorted out the CD34+CD31+CD43− HEs at day 8 of OP9 co-culture. Upon replating them onto OP9 cells for further hematopoietic differentiation, HEs from GATA2 −/− hESCs produced much less CD43+ HPCs compared with WT hESCs (Fig. 2f). Nevertheless, the HEs derived from both WT and GATA2 −/− hESCs expressed typical endothelium markers such as KDR and CD144 (VE-Cadherin) (Fig. 2g). In addition, to explore their endothelial potential, we re-cultured the sorted HEs in endothelial growth medium onto a Matrigel-coated plate. In this condition, GATA2 −/− HEs underwent further endothelial differentiation that displayed a typical endothelial phenotype and formed vascular tubes, which are comparable to WT HEs (Fig. 2h). In general, our data showed that GATA2 null hESCs could develop into functional HE lineages, but undergo deficient EHT to produce HPCs.
Characterization of hematopoietic potential of GATA2 null HPCs
We then further performed comprehensive characterization of all myeloid lineages generated from GATA2 −/− hESCs/OP9 co-culture. Regarding in vivo embryonic hematopoiesis, two distinct hematopoietic programs, the primitive hematopoiesis and definitive hematopoiesis, have been demonstrated to produce different subtype blood lineages . The primitive hematopoiesis occurred early and mainly produced primitive erythroblasts and macrophages, while the definitive hematopoiesis was associated with definitive erythroid expressing adult beta-globin and pan-myeloid precursors . Both distinct programs have been detected during in vitro hESCs/OP9 co-culturing, but it remains largely challenging to precisely define and separate these two stages . In our system, we could easily detect the erythroid precursors from GATA2 −/− hESCs/OP9 co-culture, characterized by forming E-CFUs or even BFUs (Fig. 3c) with expression of CD235a and CD71, the well-established markers for in vitro-generated erythroid cells  (Fig. 3b). Furthermore, we could detect the expression of both adult globin (HBB) and embryonic globins (HBE and HBG) in erythrocytes from GATA2 −/− hESCs/OP9 (Fig. 3c). Consistently, GATA1, the critical factor known for erythroid specification, was successfully activated and detected in GATA2 −/− erythrocytes (Fig. 3d).
In contrast, myeloid lineages from GATA2 −/− hESCs exhibited significant morphological difference to those from WT hESCs (Fig. 3e). While multiple myeloid lineages such as macrophage (M-CFCs), granulocyte (G-CFCs), and pan-myeloid CFCs (CFU-Mix) were observed from WT hESCs, we only observed mononuclear cells from GATA2 −/− hESCs, which displayed macrophage morphology under a microscope (Fig. 3e). To further confirm the identity of mononuclear cells from GATA2 −/− HPCs, we performed fluorescence-activated cell sorting (FACS) analysis of these cells with specific surface markers. For these surface markers, macrophages highly express both CD11b and CD14 while granulocytes express high CD11b but low CD14; thus, they could be used to discriminate these two lineages as reported by Rafii et al. . Consistently, we showed that GATA2 −/− hESC-derived myeloid cells displayed high expression of both CD11b and CD14, demonstrating that these cells were macrophages, but not granulocytes (Fig. 3e). In another literature, Choi et al. reported that CD14 was expressed in both granulocytic and monocytic cells generated in vitro . Thus, we also examined an additional surface marker to separate these two populations, for example, CD86, which is highly expressed in monocytes/macrophages, but lowly expressed in granulocytes . We found that the GATA2 −/− hESC-derived myeloid cells exclusively expressed high level of CD86 (Fig. 3f), further demonstrating that GATA2 −/− hESCs produced macrophages (M-CFCs), not granulocytes (G-CFCs). Further, Giemsa staining confirmed the morphology for macrophage, but not granulocyte for these monocytes (Fig. 3e). Overall, these data revealed an essential role of GATA2 in regulating granulocyte generation during human ESC-modeled hematopoiesis.
SPI1 was responsible for the HPC and granulocyte defects of GATA2 −/− hESCs
Forced expression of SPI1 rescued HPC and granulocyte defects in GATA2 −/− hESCs
GATA2 −/− hESCs partially restored the granulocyte potential in the presence of Notch signaling
Currently, studies on hematopoiesis by gene knockout approach have been limited to model organisms, such as zebra fish and mice, while studies on the genetic determinants of human hematopoiesis have been confined to overexpression and knockdown in human pluripotent stem cells. In this report, we proved that human ESCs combined with gene knockout could be utilized to investigate early human hematopoiesis by demonstrating hematopoietic defects generated through deletion of a key hematopoietic transcription factor GATA2 from human ES cell line by TALEN.
The GATA family contains a series of factors that are evolutionally conserved and essential for proper development in mammals. Among them, GATA1, GATA2, and GATA3 express and function predominantly in hematopoietic lineage cells [13, 47]. These factors exhibited spatial and temporal expression patterns among different hematopoietic lineages. For example, GATA2 expression displayed broad distribution but with a prominently high level in early hematopoietic progenitors . The function of GATA factors have been extensively examined in the mouse model through various in vivo experimental approaches. It becomes more and more clear that GATA2 is required for the genesis and/or function of HSCs. However, the function of GATA factors in human hematopoiesis has not been clearly elucidated due to the limitation of embryonic materials. Here, taking the in vitro hematopoietic differentiation of human ESCs as a model, we were able to analyze the function of GATA2 in human background. Aided by TALENs to enhance genome editing, we generated human ESCs with homozygous mutation on GATA2 (GATA2 −/− hESCs). GATA2 −/− hESCs behaved similarly to their wild-type counterparts.
In contrast, regarding hematopoietic differentiation, we observed that GATA2 −/− hESCs generated much less HPCs marked by CD34+CD43+ based on the OP9 co-culture system, but retained the potential to differentiate into major types of hematopoietic lineages, such as erythrocyte and macrophage. Surprisingly, we failed to observed granulocyte differentiation from GATA2 −/− hESCs. This is very different from previous reports. In the mouse model, Tsai and de Pater et al. reported that Gata2−/− mouse ESCs are capable of producing multipotential CFCs including granulocytes albeit in a much smaller number compared to wild-type ESCs [10, 14], and Gao et al. documented the abolished CFC potential of Gata2−/− AGM cells. Our findings indicated that the function of GATA2 would be very crucial for granulocyte development in human ESC-modeled hematopoiesis (Additional file 1: Figure S5). Meanwhile, it is worthy to note that the HPCs from GATA2 −/− hESCs failed to generate any blood CFCs in the stromal-free system (Additional file 1: Figure S3), indicating that OP9 might provide additional factors or proper niche to allow CFC formation for GATA2 −/− HPCs. In addition, the presence of Notch signaling in OP9 stromal cells restored the granulocyte potential of GATA2 −/− ESCs. This finding is consistent with a previous report  and suggests that the effect of Notch signaling in GMP cells requires GATA2 and GATA2 is the downstream of Notch signaling to inhibit HPC commitment to myeloid lineage, and further highlights the important role of the interaction between the extracellular environment and intracellular gene regulation in blood cell development.
GATA2 was also found to play a role in vascular integrity. Mammoto et al. reported that knockdown of GATA2 in endothelial cells impaired vascular formation of human endothelial cells both in vitro and in vivo . Also, Kazenwadel et al. reported that GATA2 knockdown in primary lymphatic endothelial cells abolished the lymphatic endothelial cell marker expression of FOXC2, PROX1, ITGA9, VEGFR3, and ANGPT2 . However, Tsai et al. found that GATA2 −/− embryos showed apparently normal endothelial cells, vitelline vasculature, and heart on E9.5 . Consistent with the report of Tsai et al., we found that the endothelium and vascular formation potential of GATA2 −/− hESCs were normal as demonstrated by capillary structure formation and we observed no general defects in the lymphatic endothelial cell marker expression after GATA2 mutation. This may be due to a complementary mechanism which compensates for the deletion of GATA2.
At the molecular level, several factors have been reported to be direct downstream targets of GATA2 at the HSC stage. For example, SCL/Tal1, a critical factor that controls survival of HPCs at early hematopoiesis, seems to be directly activated and maintained by GATA2 in mouse models . Runx1, another important factor for normal hematopoiesis, was also directly targeted and maintained by Gata2 in mouse HPCs . However, these factors as well as some well-known hematopoietic factors were successfully activated in the absence of GATA2 upon hematopoietic differentiation of human ESCs in our system (Fig. 4c), which might explain why most blood lineages could be generated. However, SPI1, a critical gene, which has been reported to be involved in myeloid development, was significantly downregulated in GATA2 −/− HE and HPCs. Although the partial rescue effect of SPI1 may be due to the overdose effect of forced SPI1 expression, it may also imply that other candidate targets of GATA2 contribute to EHT and/or granulocyte development yet not carefully examined currently.
GATA2 −/− hESCs failed to express SPI1 upon in vitro hematopoiesis through co-culturing with OP9, and GATA2 has been reported to directly target and activate the SPI1 locus in mouse HPCs through two conserved regions . GATA2 has been reported to be involved in EHT [8, 14, 52], whereas the role of SPI1 in EHT has not been well documented. Until very recently, Adam et al. reported that Spi1 was upregulated during the EHT process , and in another milestone study, Sandler et al. reported reprogramming of human endothelial cells to transplantable hematopoietic progenitor cells by FOSB, GFI1, RUNX1, and SPI1 induction . These studies combined with our report proved that SPI1 would potentially serve as an important regulator in EHT. Nevertheless, it is still worthy to note that Gata2 might be with specific functions in SPI1 regulation in different cell types. For instance, Gata2 could bind to the Cebpa promoter, blocking Spi1 and Runx1 binding, and so prevents Cebpa gene activation for the maintenance of cellular identity of mast cells .
Previous studies on the role of SPI1 in granulopoiesis have been conflictive in some degree. Spi1 has been proved to be crucial for HSC maintenance and myeloid differentiation , and Spi1 mutant embryos exhibited multilineage defects including the impairment of granulocytes , while other studies reported that Spi1 −/− granulocyte-monocyte progenitors (GMP) can differentiate into granulocytic precursors but with further maturation impairment  and elimination of Spi1 in GMP in adult mice showed disturbed hematopoiesis with excess granulocyte production . These studies indicated specific roles of SPI1 at different stages of granulocyte development. They implied that dysfunction of SPI1 impairs HSC generation and its commitment to downstream lineages including granulocyte, while SPI1 is not essential for the differentiation of GMP to granulocyte. In our study, we showed that SPI1 could rescue the HPC generation of GATA2 −/− hESCs and the restored HPC is with granulocyte potential. These results confirmed the role of SPI1 in HPC generation and its differentiation to granulocyte in mice and further highlight its conserved role in regulating myeloid development during hematopoiesis (Additional file 1: Figure S6).
In conclusion, we reported the first study of human hematopoiesis through gene knockout and illustrated the roles of GATA2 and SPI1 in EHT and granulocyte generation in early human hematopoiesis. Particularly, we revealed the impact of interaction between Notch signaling and GATA2 on granulocyte development.
GATA2 knockout TALENs were designed as described [25, 26], and their sequences and targeting site were illustrated in Additional file 1: Figure S1. For donor construction, left and right homology arms were cloned from genomic DNA of the H1 cell line. A loxP-flanked PGK-neomycin cassette was further inserted between two homology arms in the vector pUC57. The vector is linearized by EcoRI before targeting. For targeting, 1.5 × 106 H1 cells were electroporated with 1 μg of donor DNA and 2.5 μg of each TALEN plasmid. Then, the cells were seeded on a Matrigel-coated six-well plate in the presence of Y-27632 (10 μM, Sigma). After 2 or 3 days, positive clones were selected by G418 (100 μg/ml, Sigma). Further verifications were carried out by genomic PCR and Southern blot. All primers referred are listed in Additional file 1: Table S1.
Hematopoietic colony-forming assays
Hematopoietic colony-forming assays were performed in 35-mm culture dishes (Stem Cell Technologies, Inc.) using 1 ml per dish of MethoCult™ H4435 enriched medium (Stem Cell Technologies, Inc.) mixed with cells of a certain number according to the manufacturer’s instructions. Colonies were counted on days 14–16 and picked individually, washed in FACS buffer, and spun onto slides with a cytospin apparatus (TXD-3). The cells were then fixed and processed with Wright-Giemsa staining.
FACS analysis and cell sorting
For GFP fluorescence analysis, cells were trypsinized and suspended in FACS buffer (PBS with 2 % FBS (ExCell)) directly for detection by C6 (BD Accuri). For cell surface antigen analysis, cells were stained with antibody cocktail in FACS buffer at 4 °C for 15 to 30 min after trypsinization. Specifically, to analyze day 9 subsets from co-culture, cells were stained with CD43-FITC, CD31-PE, CD34-PerCP-Cy5.5, and TRA-1-85-APC. To analyze and sort HEs in day 8 and HPCs in day 9 of co-culture, CD34+ cells were primarily isolated by MACS (Miltenyi Biotech) and subsequently sorted using a FACSAria cell sorter (BD Biosciences) with cells stained by CD43-FITC, CD31-PE, CD34-PerCP-Cy5.5, and TRA-1-85-APC. To analyze endothelial markers expressed on HEs, cultured HEs were stained by CD144-PE and KDR-PE, respectively. To analyze colony-forming unit (CFU) surface makers, CFU-E were stained with CD235a-PE and CD71a APC; CFU-G/M/GM or myeloid cells derived from HPCs co-cultured with OP9/OP9-DL1 were stained with CD11b-FITC, CD33-PE, CD14-PerCP-Cy5.5, CD13-APC or CD11b-FITC, CD86-PE, CD14-PerCP-Cy5.5; CFU-Mix cells were stained with CD235a-PE, CD13-PerCP-Cy5.5, and CD71a-APC. To analyze the surface maker expressed on pluripotent stem cells, they were stained with no-conjugated primary SSEA-4, TRA-1-60, and TRA-1-81 antibody, respectively, and further stained with species-specific secondary antibodies conjugated to Alexa Fluor® 448 (Invitrogen). For intracellular antigen OCT4, PAX6, and NESTIN analysis, cell fixation and permeabilization were performed before antibody incubation and then cells were stained with primary and secondary antibodies as SSEA4. Particularly, for cell sorting, after staining with antibodies, cells were further stained with DAPI excluding dead cells; the purity of sorted fractions was more than 97 % as tested by FACS. All antibodies used for FACS analysis are listed in Additional file 1: Table S2.
Additional methods are listed in the Additional file 1.
The RNA-Seq data are available in the Gene Expression Omnibus database [accession number: GSE69797].
endothelial to hematopoietic transition
granulocyte and macrophage
human embryonic stem cells
hematopoietic progenitor cells
hematopoietic stem cells
We thank Dr. Thomas C. Sudhof for offering the Dox-inducible lentivirus and the members in our lab for the kindly help. Also, we thank Dr. Andrew Hutchins for the instruction in the preparation of this manuscript. This work was supported by the following: National Basic Research Program of China, 973 Program of China (2012CB966503, 2011CB965204, 2014CB964604), “Strategic Priority Research Program” of the Chinese Academy of Sciences Grant No. XDA01020202, National Natural Science Foundation of China (31371514, 31200970, 81301340), National Natural Science Foundation-Guangdong Joint Fund No. U1132005, National S&T Major Special Project on Major New Drug Innovation, Grant No. 2011ZX09102010, “Hundred Talents Program” of Chinese Academy of Sciences (to Dr.G Pan), and the Equipment Function Development & Technology Innovation Project of the Chinese Academy of Sciences (Grant Nos. yg2012049, yg2011082, and yg2011083).
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- Pimanda JE, Ottersbach K, Knezevic K, Kinston S, Chan WY, Wilson NK, et al. Gata2, Fli1, and Scl form a recursively wired gene-regulatory circuit during early hematopoietic development. Proc Natl Acad Sci U S A. 2007;104(45):17692–7. doi:10.1073/pnas.0707045104.PubMed CentralPubMedView ArticleGoogle Scholar
- Weissman IL. Stem cells: units of development, units of regeneration, and units in evolution. Cell. 2000;100(1):157–68. doi:10.1016/S0092-8674(00)81692-X.PubMedView ArticleGoogle Scholar
- Guo G, Luc S, Marco E, Lin TW, Peng C, Kerenyi MA, et al. Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire. Cell Stem Cell. 2013;13(4):492–505. doi:10.1016/j.stem.2013.07.017.PubMedView ArticleGoogle Scholar
- Sugimura R, He XC, Venkatraman A, Arai F, Box A, Semerad C, et al. Noncanonical Wnt signaling maintains hematopoietic stem cells in the niche. Cell. 2012;150(2):351–65. doi:10.1016/j.cell.2012.05.041.PubMed CentralPubMedView ArticleGoogle Scholar
- Reya T, Clevers H. Wnt signalling in stem cells and cancer. Nature. 2005;434(7035):843–50. doi:10.1038/Nature03319.PubMedView ArticleGoogle Scholar
- Mouthon MA, Bernard O, Mitjavila MT, Romeo PH, Vainchenker W, Mathieu-Mahul D. Expression of tal-1 and GATA-binding proteins during human hematopoiesis. Blood. 1993;81(3):647–55.PubMedGoogle Scholar
- Ling KW, Ottersbach K, van Hamburg JP, Oziemlak A, Tsai FY, Orkin SH, et al. GATA-2 plays two functionally distinct roles during the ontogeny of hematopoietic stem cells. J Exp Med. 2004;200(7):871–82. doi:10.1084/Jem.20031556.PubMed CentralPubMedView ArticleGoogle Scholar
- Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, et al. Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators. Nat Commun. 2014;5:4372. doi:10.1038/ncomms5372.PubMed CentralPubMedView ArticleGoogle Scholar
- Pereira C-F, Chang B, Qiu J, Niu X, Papatsenko D, Hendry Caroline E et al. Induction of a hemogenic program in mouse fibroblasts. Cell Stem Cell. 2013. doi:10.1016/j.stem.2013.05.024.
- Tsai FY, Keller G, Kuo FC, Weiss M, Chen JZ, Rosenblatt M, et al. An early haematopoietic defect in mice lacking the transcription factor GATA-2. Nature. 1994;371(6494):221–6. doi:10.1038/371221a0.PubMedView ArticleGoogle Scholar
- Minegishi N, Suzuki N, Yokomizo T, Pan XQ, Fujimoto T, Takahashi S, et al. Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos. Blood. 2003;102(3):896–905. doi:10.1182/blood-2002-12-3809.PubMedView ArticleGoogle Scholar
- Lugus JJ, Chung YS, Mills JC, Kim SI, Grass J, Kyba M, et al. GATA2 functions at multiple steps in hemangioblast development and differentiation. Development. 2007;134(2):393–405. doi:10.1242/dev.02731.PubMedView ArticleGoogle Scholar
- Tsai F, Orkin S. Transcription factor GATA-2 is required for proliferation/survival of early hematopoietic cells and mast cell formation, but not for erythroid and myeloid terminal differentiation. Blood. 1997;89(10):3636–43.PubMedGoogle Scholar
- de Pater E, Kaimakis P, Vink CS, Yokomizo T, Yamada-Inagawa T, van der Linden R, et al. Gata2 is required for HSC generation and survival. J Exp Med. 2013;210(13):2843–50. doi:10.1084/jem.20130751.PubMed CentralPubMedView ArticleGoogle Scholar
- Ikonomi P, Rivera C, Riordan M, Washington G, Schechter A, Noguchi C. Overexpression of GATA-2 inhibits erythroid and promotes megakaryocyte differentiation. Exp Hematol. 2000;28(12):1423–31. doi:10.1016/s0301-472x(00)00553-1.PubMedView ArticleGoogle Scholar
- Rodrigues NP, Boyd AS, Fugazza C, May GE, Guo YP, Tipping AJ, et al. GATA-2 regulates granulocyte-macrophage progenitor cell function. Blood. 2008;112(13):4862–73. doi:10.1182/blood-2008-01-136564.PubMedView ArticleGoogle Scholar
- Tipping A, Pina C, Castor A, Hong D, Rodrigues N, Lazzari L, et al. High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle. Blood. 2009;113(12):2661–72. doi:10.1182/blood-2008-06-161117.PubMedView ArticleGoogle Scholar
- Persons D, Allay J, Allay E, Ashmun R, Orlic D, Jane S, et al. Enforced expression of the GATA-2 transcription factor blocks normal hematopoiesis. Blood. 1999;93(2):488–99.PubMedGoogle Scholar
- Vodyanik MA, Bork JA, Thomson JA, Slukvin II. Human embryonic stem cell-derived CD34(+) cells: efficient production in the coculture with OP9 stromal cells and analysis of lymphohematopoietic potential. Blood. 2005;105(2):617–26. doi:10.1182/blood-2004-04-1649.PubMedView ArticleGoogle Scholar
- Woods NB, Parker AS, Moraghebi R, Lutz MK, Firth AL, Brennand KJ, et al. Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines. Stem Cells. 2011;29(7):1158–64. doi:10.1002/stem.657.PubMed CentralPubMedView ArticleGoogle Scholar
- Chanda B, Ditadi A, Iscove NN, Keller G. Retinoic acid signaling is essential for embryonic hematopoietic stem cell development. Cell. 2013;155(1):215–27. doi:10.1016/j.cell.2013.08.055.PubMedView ArticleGoogle Scholar
- Sturgeon CM, Ditadi A, Clarke RL, Keller G. Defining the path to hematopoietic stem cells. Nat Biotechnol. 2013;31(5):416–8. doi:10.1038/nbt.2571.PubMedView ArticleGoogle Scholar
- Vodyanik MA, Thomson JA, Slukvin II. Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures. Blood. 2006;108(6):2095–105. doi:10.1182/blood-2006-02-003327.PubMed CentralPubMedView ArticleGoogle Scholar
- Kennedy M, Awong G, Sturgeon CM, Ditadi A, Lamotte-Mohs R, Zuniga-Pflucker JC, et al. T lymphocyte potential marks the emergence of definitive hematopoietic progenitors in human pluripotent stem cell differentiation cultures. Cell Rep. 2012;2(6):1722–35. doi:10.1016/j.celrep.2012.11.003.PubMedView ArticleGoogle Scholar
- Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F. A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012;7(1):171–92. doi:10.1038/nprot.2011.431.PubMed CentralPubMedView ArticleGoogle Scholar
- Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res. 2011;39(12), e82. doi:10.1093/nar/gkr218.PubMed CentralPubMedView ArticleGoogle Scholar
- Hockemeyer D, Wang HY, Kiani S, Lai CS, Gao Q, Cassady JP, et al. Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol. 2011;29(8):731–4. doi:10.1038/Nbt.1927.PubMed CentralPubMedView ArticleGoogle Scholar
- Ma N, Liao B, Zhang H, Wang L, Shan Y, Xue Y, et al. Transcription activator-like effector nuclease (TALEN)-mediated gene correction in integration-free beta-thalassemia induced pluripotent stem cells. J Biol Chem. 2013;288(48):34671–9. doi:10.1074/jbc.M113.496174.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang L, Menendez P, Cerdan C, Bhatia M. Hematopoietic development from human embryonic stem cell lines. Exp Hematol. 2005;33(9):987–96. doi:10.1016/j.exphem.2005.06.002.PubMedView ArticleGoogle Scholar
- Chen MJ, Yokomizo T, Zeigler BM, Dzierzak E, Speck NA. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature. 2009;457(7231):887–91. doi:10.1038/nature07619.PubMed CentralPubMedView ArticleGoogle Scholar
- Tavian M, Biasch K, Sinka L, Vallet J, Peault B. Embryonic origin of human hematopoiesis. Int J Dev Biol. 2010;54(6–7):1061–5. doi:10.1387/ijdb.103097mt.PubMedView ArticleGoogle Scholar
- Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, et al. TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy. Cell Res. 2011. doi:10.1038/cr.2011.138.Google Scholar
- Rafii S, Kloss CC, Butler JM, Ginsberg M, Gars E, Lis R, et al. Human ESC-derived hemogenic endothelial cells undergo distinct waves of endothelial to hematopoietic transition. Blood. 2013;121(5):770–80. doi:10.1182/blood-2012-07-444208.PubMedView ArticleGoogle Scholar
- Wang L, Liu T, Xu L, Gao Y, Wei Y, Duan C, et al. Fev regulates hematopoietic stem cell development via ERK signaling. Blood. 2013;122(3):367–75. doi:10.1182/blood-2012-10-462655.PubMedView ArticleGoogle Scholar
- Bertrand JY, Chi NC, Santoso B, Teng S, Stainier DY, Traver D. Haematopoietic stem cells derive directly from aortic endothelium during development. Nature. 2010;464(7285):108–11. doi:10.1038/nature08738.PubMed CentralPubMedView ArticleGoogle Scholar
- Palis J, Robertson S, Kennedy M, Wall C, Keller G. Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse. Development. 1999;126(22):5073–84.PubMedGoogle Scholar
- Timmermans F, Velghe I, Vanwalleghem L, De Smedt M, Van Coppernolle S, Taghon T, et al. Generation of T cells from human embryonic stem cell-derived hematopoietic zones. J Immunol. 2009;182(11):6879–88. doi:10.4049/jimmunol.0803670.PubMedView ArticleGoogle Scholar
- Choi KD, Vodyanik MA, Slukvin II. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors. J Clin Invest. 2009;119(9):2818–29. doi:10.1172/JCI38591.PubMed CentralPubMedView ArticleGoogle Scholar
- David S, Kroner A. Repertoire of microglial and macrophage responses after spinal cord injury. Nat Rev Neurosci. 2011;12(7):388–99. doi:10.1038/nrn3053.PubMedView ArticleGoogle Scholar
- Kastner P, Chan S. PU.1: a crucial and versatile player in hematopoiesis and leukemia. Int J Biochem Cell Biol. 2008;40(1):22–7. doi:10.1016/j.biocel.2007.01.026.PubMedView ArticleGoogle Scholar
- Kim HG, de Guzman CG, Swindle CS, Cotta CV, Gartland L, Scott EW, et al. The ETS family transcription factor PU.1 is necessary for the maintenance of fetal liver hematopoietic stem cells. Blood. 2004;104(13):3894–900. doi:10.1182/blood-2002-08-2425.PubMedView ArticleGoogle Scholar
- Sandler VM, Lis R, Liu Y, Kedem A, James D, Elemento O, et al. Reprogramming human endothelial cells to haematopoietic cells requires vascular induction. Nature. 2014;511(7509):312–8. doi:10.1038/nature13547.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, et al. Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron. 2013;78(5):785–98. doi:10.1016/j.neuron.2013.05.029.PubMed CentralPubMedView ArticleGoogle Scholar
- Zhang P, Zhang X, Iwama A, Yu C, Smith KA, Torbett B, et al. PU.1 inhibits GATA-1 function by blocking GATA-1 DNA binding: potential role in normal hematopoiesis and leukemogenesis. Blood. 1999;94(10):683a-a.Google Scholar
- de Pooter RF, Schmitt TM, de la Pompa JL, Fujiwara Y, Orkin SH, Zuniga-Pflucker JC. Notch signaling requires GATA-2 to inhibit myelopoiesis from embryonic stem cells and primary hemopoietic progenitors. J Immunol. 2006;176(9):5267–75. doi:10.4049/jimmunol.176.9.5267.PubMedView ArticleGoogle Scholar
- Schroeder T, Kohlhof H, Rieber N, Just U. Notch signaling induces multilineage myeloid differentiation and up-regulates PU.1 expression. J Immunol. 2003;170(11):5538–48. doi:10.4049/jimmunol.170.11.5538.PubMedView ArticleGoogle Scholar
- Leonard M, Brice M, Engel J, Papayannopoulou T. Dynamics of GATA transcription factor expression during erythroid differentiation. Blood. 1993;82(4):1071–9.PubMedGoogle Scholar
- Mammoto A, Connor KM, Mammoto T, Yung CW, Huh D, Aderman CM, et al. A mechanosensitive transcriptional mechanism that controls angiogenesis. Nature. 2009;457(7233):1103–8. doi:10.1038/nature07765.PubMed CentralPubMedView ArticleGoogle Scholar
- Kazenwadel J, Secker GA, Liu YJJ, Rosenfeld JA, Wildin RS, Cuellar-Rodriguez J, et al. Loss-of-function germline GATA2 mutations in patients with MDS/AML or MonoMAC syndrome and primary lymphedema reveal a key role for GATA2 in the lymphatic vasculature. Blood. 2012;119(5):1283–91. doi:10.1182/blood-2011-08-374363.PubMed CentralPubMedView ArticleGoogle Scholar
- Nottingham WT, Jarratt A, Burgess M, Speck CL, Cheng JF, Prabhakar S, et al. Runx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer. Blood. 2007;110(13):4188–97. doi:10.1182/blood-2007-07-100883.PubMed CentralPubMedView ArticleGoogle Scholar
- Chou ST, Khandros E, Bailey LC, Nichols KE, Vakoc CR, Yao Y, et al. Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate. Blood. 2009;114(5):983–94. doi:10.1182/blood-2009-03-207944.PubMed CentralPubMedView ArticleGoogle Scholar
- Gao X, Johnson KD, Chang YI, Boyer ME, Dewey CN, Zhang J, et al. Gata2 cis-element is required for hematopoietic stem cell generation in the mammalian embryo. J Exp Med. 2013;210(13):2833–42. doi:10.1084/jem.20130733.PubMed CentralPubMedView ArticleGoogle Scholar
- Wilkinson AC, Kawata VK, Schutte J, Gao X, Antoniou S, Baumann C, et al. Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification. Development. 2014;141(20):4018–30. doi:10.1242/dev.115709.PubMed CentralPubMedView ArticleGoogle Scholar
- Sy O, Moriguchi T, Noguchi Y, Ikeda M, Kobayashi K, Tomaru N, et al. GATA2 is critical for the maintenance of cellular identity in differentiated mast cells derived from mouse bone marrow. Blood. 2015;125(21):3306–15. doi:10.1182/blood-2014-11-612465.View ArticleGoogle Scholar
- Iwasaki H, Somoza C, Shigematsu H, Duprez EA, Iwasaki-Arai J, Mizuno S, et al. Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation. Blood. 2005;106(5):1590–600. doi:10.1182/blood-2005-03-0860.PubMed CentralPubMedView ArticleGoogle Scholar
- Scott EW, Simon MC, Anastasi J, Singh H. Requirement of transcription factor Pu.1 in the development of multiple hematopoietic lineages. Science. 1994;265(5178):1573–7. doi:10.1126/science.8079170.PubMedView ArticleGoogle Scholar
- DeKoter RP, Walsh JC, Singh H. PU.1 regulates both cytokine-dependent proliferation and differentiation of granulocyte/macrophage progenitors. Embo J. 1998;17(15):4456–68. doi:10.1093/emboj/17.15.4456.PubMed CentralPubMedView ArticleGoogle Scholar
- Dakic A, Metcalf D, Di Rago L, Mifsud S, Wu L, Nutt SL. PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesis. J Exp Med. 2005;201(9):1487–502. doi:10.1084/Jcm.20050075.PubMed CentralPubMedView ArticleGoogle Scholar