Fig. 1

Distinct properties in human HSPCs at different developmental stages. A Scheme of analyzed HSPC samples at various stages of human development. The analyzed single cell transcriptome data of CD34 + CD38-Lin- HSPCs are from yolk sac (YS, CS10/CS11)、AGM (CS13), fetal liver (FL, week7-week17), umbilical cord blood (UCB) and mobilized in peripheral blood (PB). B Uniform manifold and projection (UMAP) plot of single cell transcriptomes of HSPCs from various stages indicated by different colors. C Plots of blood lineage gene expressions in HSPCs at different stages. Color indicate expression level (TPM, Log-scaled). D Pseudotime developmental path analysis on HSPCs at different stages. UMAP plot is ordered by pseudotime score as colored. E Heatmap for gene clusters with expression changes along the pseudotime progression. Four clusters based on changed pattern are indicated by different colors. The scaled expression level (TPM, z-normalized) of each cluster are shown. F Schematic plot of dynamic changes of each cluster genes across pseudotime. Lines indicate mean values of scaled expression levels (TPM, z-normalized). G Top Gene Ontology (GO) terms enriched in different gene clusters. Terms of critical HSC functions are highlighted. H UMAP plot of predicated cell cycle phases indicted by different colors. I Cell cycle phases of HSPCs from different stages. J GSEA score plots of the gene sets related to cell cycle and DNA replication in HSPCs at different stages