Fig. 2

Metabolism features in human HSPCs at different developmental stages. A GSEA score plots of the gene sets with oxidative phosphorylation or glycolysis in HSPCs at different stages. B Dot plot of the expression level of selected metabolism genes in different HSPCs. Color indicates the average of standardized gene expression (TPM, Log-scaled) and size of the dot indicates the percentage of cells expressing this gene in each group. C Heatmap of GSEA score in different HSPCs for the indicated gene sets. GSEA scores indicated by NES number are shown and differentially colored. D Dot plot displaying the expression level of selected genes involved in PI3K-AKT-MTOR and AMPK pathway in different HSPCs. Color indicates the scaled mean expression level (TPM, Log-scaled) and the size of the dot indicates the percentage of cells expressing the given gene. E Heatmap for expressions of gene clusters related to autophagy and FOXO pathway along the pseudotime progression. Color displays the scaled expression level (TPM, z-normalized)