Fig. 3

Differential lineage potentialities in HSPCs from different developmental stages. A Heatmap of GSEA score in different HSPCs for the indicated HSC lineage gene sets selected from published papers. GSEA scores indicated by NES number are shown and differentially colored. B-C Lineage potentiality genes display differential dynamics in HSCs across pseudotime developmental path. Megakaryocyte and erythrocyte potentiality genes are down-regulated (B) while the T cell potentiality genes are up-regulated (C). Bar plots indicate the enrichment of selected GO terms; dot plots indicate the expression level of selected genes involved in the biological process as shown on the left; feature plots show the expressions of selected genes in different HSCs. Color indicate expression level (TPM, Log-scaled). D Expressions of RNAi and interferon genes in different HSCs. Color indicate expression level (TPM, Log-scaled). E Expressions of MHC and selected immune privilege genes in different HSCs. Color indicates the scaled mean expression level (TPM, Log-scaled) and the size of the dot indicates the percentage of cells expressing the given gene