Fig. 5

Human iPSCs derived HSPCs are close to early embryonic stage HSPCs. A UMAP plot of the single cell transcriptomes of human iPSCs derived HSPCs (iHSPCs) and other indicated human HSPCs. B Pearson ‘s correlation plot of indicated HSPCs. Co-relation value scores are indicated. C Predicated cell cycle stages indicated HSPCs. D GSEA score plots of the gene sets with cell cycle or oxidative phosphorylation in iHSPCs. E Heatmap of GSEA score in indicated HSPCs for the indicated HSC lineage gene sets selected from published papers. GSEA scores indicated by NES number are shown and differentially colored.. F UMAP plot based on the regulon activity score colored by samples. G Heatmap of scaled regulon activity of indicated HSPCs. Regulators shown in the plot are selected from Fig. 4B. H Distribution of regulon modules in iHSPCs. I Expression of indicated genes in iHSPCs. Color indicates the scaled mean expression level (TPM, Log-scaled) and the size of the dot indicates the percentage of cells expressing the given gene