Fig. 6

Expression of key TFs promotes iHSPC functions. A Expression of indicated genes in different HSPCs. Color indicates the scaled mean expression level (TPM, Log-scaled) and the size of the dot indicates the percentage of cells expressing the given gene. B Regulon activity and expressions of indicated regulators. C Scheme of the experiment design. D FACS analysis of the indicated iHSPCs with expression of the indicated TFs. E Expression of indicated TFs in each indicated iHSPCs by qRT-PCR assay. F CFU analysis on indicated iHSPCs with expression of key TFs. G Analysis the NK differentiation on indicated iHSPCs. iHSPCs are co-cultured with MS5 cells and NK cells are analyzed by FACS based on CD45⁺CD56⁺. The number of NK cell generated per seeded 2X10⁴ HSPCs are shown. H Analysis the T differentiation on indicated iHSPCs. iHSPCs are co-cultured with OP9-DL4 cells and T progenitor cells are analyzed by FACS based on CD45⁺CD7⁺ or CD5⁺ at 2 weeks differentiation. T mature CD8⁺CD4⁻ cells are analyzed at 4 weeks differentiation. The number of indicated T progenitor or mature T cells generated per seeded 2X10⁴ HSPCs are shown. The significance level was determined by unpaired two-tailed Student’s t tests. *, p < 0.05; **, p < 0.01; ***, p < 0.001. The data represent the mean + SEM from three independent repeats (n = 3). All error bars throughout the figure represent the SEM from three independent replicates (n = 3)