Figure 2
From: Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells
Oxygen flux in permeabilized wild-type mesenchymal stem cells (WT-MSC) and MRL/MpJ mesenchymal stem cells (MRL-MSC). (A) Routine respiration in MiR05 (RMiR05) in intact MSCs. State 2 oxygen consumption through Complex I supported by glutamate and malate (VCI) in digitoin-permabilized MSCs. State 3 or maximal oxygen consumption through Complex I supported by glutamate and malate (VMAX-CI) in digitoin-permabilized MSCs. State 3 or maximal oxygen consumption through Complex II supported by succinate (VMAX-CII) in digitoin-permabilized MSCs. (B) Given MSCs are transplanted into the infarcted heart, isolated, primary cardiac myocyte oxygen utilization is provided as a metabolic reference state. Cardiac myocyte state 2 oxygen consumption through Complex I supported by glutamate and malate (VCI) in digitoin-permabilized cardiac myocytes. State 3 or maximal oxygen consumption through Complex I supported by glutamate and malate (VMAX-CI) in digitoin-permabilized cardiac myocytes. State 3 or maximal oxygen consumption through Complex II supported by succinate (VMAX-CII) in digitoin-permabilized cardiac myocytes. (C) Acceptor control ratio (ACR; defined as VMAX-CI/VCI). n = 6-9, data are mean ± S.E.M. *p < 0.05 vs. WT-MSC. †p < 0.05 vs. MRL-MSC.