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Figure 3 | Cell Regeneration

Figure 3

From: Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells

Figure 3

Effects of 48-hour glucose deprivation and ischemia on wild-type mesenchymal stem cell (WT-MSC) and MRL/MpJ mesenchymal stem cell (MRL-MSC) apoptosis. (A) Representative fluorescence-activated cell sorting analysis of apoptotic cells after mitosense red and calcein, AM staining. Healthy, viable MSCs are mitosense red+/calcein, AM+. The mitosense red-/calcein, AM+ MSCs are in the early apoptotic phase, whereas the mitosense red-/calcein, AM+ MSCs are in the late apoptotic/cell death phase. (B) Quantitation of MSC early apoptosis induced by 48 hr glucose deprivation and ischemia with mitosense red and calcein, AM staining. The graph shows the percentage of all MSCs that are mitosense red-/calcein, AM+ MSCs after glucose deprivation and ischemia. (C) Quantitation of MSC late apoptosis/cell death induced by 48 hr glucose deprivation and ischemia with mitosense red and calcein, AM staining. The graph shows the percentage of all MSCs that are mitosense red-/calcein, AM- MSCs after glucose deprivation and ischemia. n = 6-7 independent experiments, data are mean ± S.E.M. *p < 0.05 vs. WT-MSC Control. †p < 0.05 vs. MRL-MSC Control. ¶p < 0.05 vs. WT-MSC of identical experimental conditions. §p < 0.05 vs. WT-MSC Glucose Deprivation. #p < 0.05 vs. MRL-MSC Glucose Deprivation.

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