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Using mouse models to study function of transcriptional factors in T cell development
Cell Regenerationvolume 1, Article number: 8 (2012)
Laboratory mice have widely been used as tools for basic biological research and models for studying human diseases. With the advances of genetic engineering and conditional knockout (CKO) mice, we now understand hematopoiesis is a dynamic stepwise process starting from hematopoietic stem cells (HSCs) which are responsible for replenishing all blood cells. Transcriptional factors play important role in hematopoiesis. In this review we compile several studies on using genetic modified mice and humanized mice to study function of transcriptional factors in lymphopoiesis, including T lymphocyte and Natural killer (NK) cell development. Finally, we focused on the key transcriptional factor Bcl11b and its function in regulating T cell specification and commitment.
Lab mice are invaluable tools in modern biomedical research because of its short generation time, small size and prolificacy in breeding. A pure genetic background of the inbred mouse strain greatly improves the reproducibility of experiments, as individuals from the same mouse strain are genetically identical. Currently, all the common inbred strains in labs have been inbred for at least 80 generations since their original isolation; thus the genomes of all siblings of the same inbred line are essentially identical . These inbred mouse strains provide good platforms for studying the immune system and modeling human immune disease [2–4]. In thymus, hematopoietic cells undergo several developmental processes to give rise to variety of T subsets which is one of the central players in orchestrating immune responses. Rothenberg and Taku Naito have reviewed a combination of transcription factors, including E2A, GATA3 and TCF1, which are essential to initiate T cell differentiation program . However the key factor of T lineage specification and commitment in vivo is to be understood. Recently, Bcl11b, a transcriptional factor, was identified to be essential for T cell development and for the maintenance of T cell identity [6–8]. Deletion of Bcl11b in T cells can cause these cells to reprogramme into NK cells in mice . Our goal here is to give an introduction on the function of transcriptional factors, especially Bcl11b, in T cell development
Genetic manipulation of the mouse genome
Over-expression of targeted genes—gain-of-function
A most breakthrough advantage of using the mouse to study the immune system and to model human disease is the availability of a range of genetic technologies. In 1981, several groups produced transgenic mice by injecting transgenic DNA into mouse pronuclei [9, 10]. Importantly, DNA introduced into the mouse genome by this method results in establishment of the transgene in the germ line. This technology offers scientists the opportunities to perform gain-of-function studies for specific genes in the mouse model. Currently, DNA fragments can be conveniently inserted into the host cell genome by virus  or transposon system, such as Sleeping Beauty  and PiggyBac [13, 14]. Woltien K and his colleagues successfully reprogrammed murine and human embryonic fibroblasts to induced pluripotent stem cell (iPS) using doxycycline-inducible transcription factors delivered by PB transposition . However, the copy number and the genomic position of the transgene that integrates into the mouse genome vary in different operation. Therefore, it is difficult to control the expression levels or patterns of the transgene . In addition, except bacterial artificial chromosome transgenes , transgenes usually are not large enough to contain all of the cis-acting elements for recapitulating the endogenous gene expression patterns . In a word, loss-of-function assays are usually recruited to study gene functions besides gain-of-function assays.
Silent target genes—loss-of-function
In 1981, pluripotent mouse embryonic stem cells (ES) were isolated from the inner cell mass of 3.5 days post-coitum (dpc) wild type mouse embryos [19, 20]. Later, it was demonstrated that these ES cells were able to contribute to the germ line in chimera mice derived from ES cells, even after genome modification by retrovirus [21, 22]. Precise manipulation of the mouse genome was achieved by demonstrating that homologous recombination works efficiently in mouse ES cells [23, 24]. Thus a combination of mouse ES cell manipulation and homologous recombination technologies gave birth to ‘gene targeting’ which has revolutionized mouse genetics. With birth of genetically engineered ‘knockout’ mice, target mutations have been introduced to many loci to study gene functions in the immunity. In 1990, Smithies’ and Jaenisch’s group independently generated β2-microglobulin knockout mice in which expression of major histocompatibility complex (MHC) class I molecules was abolished. The mutant mice developed normally but had severely reduced number of CD8+ cytotoxic T lymphocytes (CTLs), indicating that MHC class I molecules are required for the selection of MHC class-I-restricted CD8+ T cells and for antigen recognition by these cells, but not necessary for T cell development in general [25, 26]. It is now feasible to knockout every single gene in the mouse and observe phenotype in this knockout mice (Figure 1) .
Conventionally, homologous recombination is employed in gene targeting to insert a designed mutation into the homologous recombination loci in mouse ES cells. These ES cells are totipotent and when injected into mouse blastocytes, these cells can differentiate into all cell types of a chimeric mouse . In addition, homologues knockout of some important gene, such as the house keeping gene, leads to embryonic death , exposing the defect of knockout technology, as we lost the opportunist to study function of these genes in adult mice. Conditional knockout mice approaches, often using the Cre-loxP site-specific recombination system, were developed to overcome the embryonic lethality problem and to investigate gene function temporally and spatially [30, 31]. For example, T cell development is arrested at early stages in Notch1 and Gata3 adult CKO mice, demonstrating the importance of these two genes for T cell development . However, either Notch1 or Gata3 deleted mice are died during embryonic period [33, 34]. Similarly, conditional knock out of Bcl11b in double negative (DN) 3 T lymphocytes induce DN3 T cell differentiate to NK cell , but Bcl11b in conventional knockout mice die within the first day after birth .
Although the mouse is instrumental in elucidating key processes in the immune system and revealing molecular mechanisms of immune diseases [36, 37], ethical issues and significant species differences between mouse and human constrain researchers to study human immune system in vivo. Humanized mice, or mouse-human chimaeras, have been developed to overcome these constraints and are now an important research tool for the in vivo study of human hematopoietic system. Humanized mice are classified into two kinds: one is immune deficient mice engrafted with hematopoietic cells or tissues, that the other is transgenically expressing human genes. And the latter have been previously reviewed . In this review, humanized mice refer to immune deficient mice that are engrafted with hematopoietic cells or tissues. Many important research advances have been made using humanized mice as models to study human hematopoietic system. For example, Faiyaz Notta and his colleagues purified so far the most accurate human HSC Lin−CD34+C D38−CD45RA−Thy1+RholoCD49f+ at single cell resolution in immune deficient mice .
Several mouse strains have so far been used as recipients of human hematopoietic cells. Scid (severe combined immune deficiency) mice carry a spontaneous loss of function mutation of the Prkcdscid gene, or scid mutation which results in severely deficient in T and B lymphocytes and aberrant adaptive immune system . Consequently, scid mice can engraft human hematopoietic stem cells/progenitors, mature lymphocytes and tumor cells without rejection . Though scid mice were proved to be successful, the engraftment efficiency was usually low due to high levels of innate immunity response in mice . To overcome this problem, Shultz LD and his colleagues  generated the NOD-Scid mice (NOD/LtSzPrKdcscid /PrKdscid) strain by introducing the scid mutation from CB17-Scid mice into the NOD strain mice that contain reduced NK cell activity . However, NOD-Scid mice are still not good enough in xenograft for the residual innate immunity . The interleukin-2 receptor γ-chain (Il2rg) locus is an essential component of the receptor for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21 . Loss-of-function mutation at the IL-2R γ-chain results in severe impairments in T- and B-cell development and function, and completely prevents NK-cell development . Two sub-strains—NOD/ShiLtSz-scid/IL2R γnull (-NSG) and NOD/ShiJic-scid/IL2R γnull (NOG) which can engraft higher human grafts were generated by introducing Il2rg-null mutations to NOD- Scid mouse independently. [47, 48]. Both NSG and NOG strains are widely used for studying human hematopoiesis and leukemiagenesis .
Hematopoiesis is the dynamic and complex developmental process of the formation of new blood cells, including red blood cells (erythrocytes), white blood cells (leukocytes), and platelets . For mammalians, hematopoiesis can be divided into two periods, the primitive hematopoiesis and definitive hematopoiesis. Primitive hematopoiesis is referred as hematopoiesis taking place prior to the development of the fetal liver. The term definitive hematopoiesis is used to describe blood formation after the formation of the fetal liver. In the mouse embryos, primitive hematopoiesis formed at 8.0 days postconception (dpc 8) in the yolk sac blood islands. During embryonic development, the location of hematopoiesis shifts from the aorta-gonad-mesonephros (AGM) region to the fetal liver . After birth, the hematopoiesis center migrates from the fetal liver to the bone marrow (BM), where the special microenvironment (the endosteal niche) supports hematopoiesis .
HSCs are a heterogeneous pluripotent population and are composed of long-term HSC and short-term HSC. Long-term HSCs are capable of self-renew for the whole life of the host, whereas short-term HSCs retain self-renewal capacity for approximately 8 weeks . Short-term HSCs proliferate and differentiate to multipotent progenitors (MPP) that give rise to common myeloid progenitors (CMP) and common lymphoid progenitors (CLP). CMP give rise to granulocyte macrophage progenitors (GMP) and megakaryocyte erythroid progenitors (MEPs) . GMP can differentiate towards neutrophils, monocytes, macrophages, eosinophils, basophils, and mast cells, while MEP generate megakaryocytes and erythrocytes . In contrast, CLP are restricted to give rise to T cells, B cells, Natural Killer (NK) cells, and some dendritic cells, but two studies suggest that some progenitors derived from CLP retain myeloid differentiation potentials (Figure 2) [56, 57]. However, cell lineage can be studied by over-expression or inactivation of some key transcriptional factors in hematopoietic lineages . Ectopic expression of Gata1 forced primary myeloid progenitors differentiate to erythroid, eosinophil, and basophil-like cell lineages, instead of macrophages and granulocytes . Similarly, expression of PU.1 and C/EBPα in early mouse T cells endows the lymphoid T cell with myeloid differentiation potential . B and T lymphocytes can be reprogrammed to myeloid cells upon over expressing PU.1, C/EBPα and/or C/EBPβ [60, 61].
T cell development
T cells are derived from the thymus and are important for adaptive immune responses . T cells are capable of recognizing antigen peptides that are bound to MHC molecules. There are two main subpopulations of T cells: CD4+ helper T and CD8+ cytotoxic T cells. CD4+ T cells recognize MHC II molecule complexes and secrete various cytokines after being activated. Conversely, CD8+ T cells recognize MHC class I molecules and exhibit cell-killing activity after activation [63, 64]. Acquired Immune Deficiency Syndrome (AIDS) provides a vivid and tragic illustration of the importance of T cells in immunity. The human immunodeficiency virus (HIV), the causative agent of AIDS, binds to the CD4 molecules which causes depletion of CD4+ T cells in AIDS patients . Without CD4+ T cells, AIDS patients become hypersusceptible to pathogens that normally inhabit in tissues without much harming, and die of the opportunistic infections .
T cell development, which happens in the thymus, involves progenitor homing, lineage specification and commitment . It also requires the intrathymic microenvironment and interactions among key transcription factors [5, 68]. In adult mice, CLPs migrate from the bone marrow to the thymus and initiate the program of T cell development . In the thymus, thymocytes are classified into three distinct maturational---double negative (DN; CD4-CD8-), double positive (DP; CD4+CD8+) and single-positive (SP; CD4-CD8+ or CD4+CD8-) (Figure 3) .
Classically, the DN population can be further subdivided into four subtypes (DN1—4) based on the level of markers CD117 (c-Kit), CD44, and CD25 expressed on the surface of the cell . The CD117+DN1 (CD44+CD25-) subsets, also known as early T cell precursors (ETP), are thought to contain not only T lineage potential but also B lineage and granulocyte-monocyte potential [56, 72]. Flt3/Flk2, a tyrosine kinase receptor, endows ETP the choice to differentiate into not only T cell, but also B cell, NK cell and macrophages . In addition, both Gata3 (GATA-binding protein 3) and Bcl11b are essential for ETP maintenance and development [7, 74]. Notch signaling is indispensable for ETP . Recently, T-cell factor 1 (TCF-1; also known as transcription factor 7, T-cell specific, TCF7) was identified as a messenger that transmit the commands from Notch signal to downstream transcriptional factor like Gata3 and Bcl11b during T cell specification and differentiation .
T cell specification occurred in the course of transition from ETP to DN2 (CD44+CD25+). During this period, several key T cell genes, such as Bcl11b, Tcf12 (HEBAlt), Gata3 and Notch1 are up-regulated. DN2 T cells notwithstanding, retain the potentials to differentiate into NK cells and myeloid cells [56, 57, 77]. ETPs and DN2 thymocytes initiate TCR gene rearrangements. However, they do not exhibit full V(D)J rearrangements or express any TCRβ or TCR γδon their cell surface, which are symbols of committed T cells. The non-T cell potentials are lost in the DN3 (CD44-CD25+) T cell stage . Some DN3 T cells successfully rearrange TCR γ- and δ-chains instead of β-chain and differentiate into γδ-T cells. The majority of T cells at DN3 stage commit to the αβ-T cell lineage by further upregulating key T cell genes and shutting down expression of genes that are important for non-T cell lineages . Co-activities of E2A and HEB are not only crucial for TCR rearrangement , but are also essential for T cells to regulate β selection, positive selection and lineage-specific gene expression at the subsequent T-cell receptor checkpoint . The DN4 (CD44-CD25-) thymocytes undergo β-selection after successful TCR βgene rearrangements and initiate the differentiation to CD4+CD8+ double positive (DP) cells .
Positive selection of the developing T-cell receptor repertoire occurs in the thymic cortex where DP cells (CD4+CD8+) selected via MHC class II and MHC class I molecules will eventually become CD4+ cells and CD8+ cells, respectively . During negative selection, that strong interaction between thymocytes and antigens would trigger Fas apoptotic signal, which in turn encourage apoptosis of the thymocytes . After positive and negative selections, the surviving thymocytes migrate to the peripheral lymphoid tissues where cytokines like IL-7 and the constant interaction between T cell and self-peptide MHC play a critical role in T cell maintenance . Moreover, thymic epithelial-cell microenvironments are indispensible for T cells selection and maintenance during immune responses . Some transcriptional factors are required in T cell maturation. For example, E protein transcription factors HEB and E2A are vital in DP to SP transition . Besides the transcriptional factors, establishment of T cell identity is also regulated by epigenetic modification. For instance, PU.1 and GATA3 that are essential for T cell development are controlled by dynamic histone modification at promoter region .
NK cell development
NK cell were first discovered by Rolf Kiessling in the 1970s, when he found a small population of large granular lymphocytes displayed cytotoxic activity against a wide range of tumor cells in the absence of any previous immunization with the tumors [87, 88]. This population of cells were named "natural killer" because of the initial notion that they did not require prior activation to kill cells. It was later shown that NK cells preferably targeted cells that expressed low levels of MHC class I molecules, a concept termed as "missing-self" recognition [89, 90]. NK cells constitute an essential component of the innate immune system, which, unlike the adaptive immune system, recognizes and responds to pathogens without requiring prior priming through clonal antigen receptors. Mice with deficiencies in NK stimulatory immune-receptors such as NKG2D and DNAM-1 are defective in tumor surveillance in models of spontaneous malignancy .
NK cell development occurs mainly in BM, although a bipotent T/NK progenitor (TNKPs) containing T- and NK-cell potentials has been identified in mouse fetal liver and fetal thymus . In BM, CLP commit to natural killer cell precursors (NKPs), which are defined as Lin-(lineage)CD122+NK1.1-DX5- . Upon CD122 expression, NKPs lose B-, T- or myeloid-cell potentials and respond to IL-15 stimulation, which promotes NK cell development . Then, NKPs further differentiate into immature NK cells that express NK1.1 in bone marrow and liver [95, 96]. Finally, immature NK cells differentiate into mature NK cells and obtain NK-cell self-tolerance by expressing inhibitory NK-cell receptors like Ly49 and CD94-NKG2. . Mature NK cells circulate in the body as defenders to maintain homeostasis and shape their weapons by producing Fasl, Trail, γInterferon- IFN-γ perforin and granzymes . Thymic NK cells express both GATA3 and CD127 (IL-7Rα). Compared to conventional NK cells, the cytotoxicity in thymic NK cells is compromised . Moreover, it was reported that NK cell progenitors (CD34+CD1a−) in human thymus that eventually differentiated into functional CD56+ NK cells express bone morphogenetic protein (BMP) receptors IA (BMPRIA), suggesting that BMP signaling pathway was required for human thymic NK development . In addition, NKp46, a NK cell marker encoded by Ncr1 gene, was found capable of blockading enhanced NK cell reactivity . Recent studies proposed that Bcl11b was a key regulator in controlling NK cell development as loss of Bcl11b induced T cell reprogrammed to NK like cell [6, 102].
The B-cell lymphoma/leukemia 11 (Bcl11) family has two members, Bcl11a and Bcl11b. Both of them are Kruppel-like C2H2 type zinc finger transcription factors . Bcl11a was first discovered as a retroviral insertion site (Evi9) in myeloid leukemia tumors in the BXH-2 mouse . Located at 2p16.1, Bcl11a is consists of 5 exons and encodes three different isoforms (Figure 4A). The first three exons of Bcl11a present in all the three isoforms. The longest isoform comprised exon 1-4, whereas the rest two common shorter isoforms exhibited alternative splicing from within exon 4 to an additional exon 5. However, the exon 5 in the isoform 2 is different from the one in the isoform 3 due to frame shift in their exon 4.
Bcl11a is also expressed in early T cell progenitors and is also important for T cell development. Germ-line deletion of Bcl11a causes neonatal lethality and an absence of B cells at the earliest B cell development stages [105, 106]. Recipient mice of Bcl11a-deficient fetal liver cells develop T cell leukemia . Genome-wide association studies in human have revealed association of the Bcl11a locus with persistent fetal hemoglobin in the adult [107, 108]. Further characterization of Bcl11a mutant mice also uncovers the key role of Bcl11a in the fetal to adult expression switch of hemoglobin . Beside of its important functions in B cell development and the switch of hemoglobin expression, Bcl11a is related to pancreatic beta-cell function, dysfunction of which leads to type 2 diabetes . In addition, a recent study shows that Bcl11a is required in neuronal morphogenesis and sensory wiring of the dorsal spinal cord .
Bcl11b, also named Rit1 (radiation-induced tumor suppressor gene 1), is the other member of the Bcl11 family in the mouse and human genomes. Bcl11b located at 14q32.2, is consists of 4 exons and encodes 2 isoforms. Compared to the isoform 1 of BCL11B that contains 894 amino acids, its isoform 2 lacks exon 3 and only have 823 amino acids. All the 6 zinc fingers are distributed within the exon 4. Alejandro Gutierrez and his colleagues find that only exon 2 and 3 are responsible for encoding the DNA binding domains . some other domains within the Bcl11b participate in protein-to-protein interactions, including histone deacetylase (HDAC1 and HDAC2) , sirtuin family protein (SIRT2) , metastasis-associated proteins (MTA1 and MTA2) , Rb-associated protein (RbAp46 and RbAp48)  and histone methyltransferase (SUV39H1)  (Figure 4B).
Bcl11b was initially identified as a tumour suppressor gene in T cells, because loss of heterogynous (LOH) contributed to the formation of thymic lymphomas in γ–ray irradiated mice [117, 118]. In addition, BCL11B is found to be involved in human T cell leukemia. A specific cryptic translocation, t(5;14)(q35.1;32.2), accrued in about one fifth of T-cell adult leukemia/lymphoma (T-ALL) patients, serves to activate expression of Hox11L2 by juxtaposition with strong T cell enhancer elements at the 3’ of the Bcl11b locus [119, 120]. Further more, a novel chromosomal aberration, inv (14) (q11.2q32.31) was reported in T-ALL samples. In this inversion, the 5’ part of Bcl11b, including exons 1-3, was joined to the TRDD3 segment of the TCR δlocus. Consequently, in-frame transcripts with truncated human BCL11B and TCRδconstant region were highly expressed in screened T-ALLs but not in normal T cells . Interestingly, though Bcl11b i s considered as a tumour suppressor gene, it is highly expressed in many human T cell tumour lines and is required for their survival. Suppression of Bcl11b by RNA interference (RNAi) causes apoptosis of these tumour cells, possibly due to a decrease of a cell-cycle inhibitor, p27, and an anti-apoptotic protein, BCL-xL. This indicates involvement of the mitochondrial apoptotic pathway. In contrast, normal mature T cells remained unaffected within the experimental time period [122, 123]. Therefore, Bcl11b could be an attractive therapeutic RNAi target in T-cell malignancies .
Function of Bcl11b in early T cells
During evolution, a homolog of Bcl11b first appeared in cartilaginous fishes. In sea lampreys, a jawless vertebrate, expression of a Bcl11b ortholog is specifically detected in VLRA+ cells that are similar to T lymphocytes in vertebrates, but not in VLRB+ cells that are similar to B lymphocytes in vertebrates . In bony fish, the Bcl11b ortholog is expressed in the thymus and positively regulates Ccr9 expression, which encodes the receptor for ccl25, a novel chemokine expressed in thymic epithelium . In both the mouse and human, Bcl11b is highly expressed in T cells [35, 119]. In the hematopoietic system, Bcl11b was absent in B cells, myeloid cells and most NK cells, but highly expressed in T cell lineage . Developmentally, its expression is tightly associated with T cell commitment. Early thymocytes, for example, start to express Bcl11b during the transition from ETP to DN2b stage, at a time when early thymocytes gradually lose non-T-cell potentials, by histone 3 methylation (H3) and demathylation , implicating that Bcl11b plays an important role in this process. Bcl11b expression is maintained at high levels in T cells beyond DN2b stages . In mice, over-expression of Bcl11b results in the differentiation of T-helper (Th) cells , whereas down-regulating or knockout of Bcl11b induces reprogram from T cell into NK-like cell . However, function of Bcl11b in human T cell requires further investigations.
Loss-of-function studies on Bcl11b in the mouse demonstrated that Bcl11b is required for early T cell development and the survival of T cells. Bcl11b homozygous mutant knockout mice die in the first few days after birth possibly due to neurological or other uncharacterized defects [35, 128, 129]. Furthermore, in this Bcl11b-deficient strain, T cell development is blocked at the DN2-DN3 stage without obvious defects in other hematopoietic lineages. A recent study using the same knockout strain shows that Bcl11b-deficient thymocytes are arrested at DN2 stage and acquires self-renewal properties in vitro . Our results, together with a study from Rothenberg’s lab, show that Bcl11b-deficient DN1 and DN2 thymocytes stop T cell development and acquire NK differentiation potentials in mouse thymus. We thus named these killer cells that were reprogrammed from T cells as induced T-to–natural killer (ITNK) cells . In Bcl11b-deficient thymocytes show impaired Vβ to Dβ rearrangements and absence expression of pre-T cell receptors (pre-TCR) complex on the cell surface . In addition, thymocytes undergo profound apoptosis in neonatal Bcl11b-deficient mice . However, apoptosis is unlikely to be the main reason for the failure of T cell development upon loss of Bcl11b, because inactivation of p53 in mutant thymocytes is not sufficient to fully restore the T cell development, even though some immature single-positive (ISP) T cells are indeed detected in Bcl11b-/- p53-/- embryos . The exact cause of T cell defects in Bcl11b-deficient mice thus remained unresolved. Taken together, Bcl11b is required for the early T cells to sustain T cell development and suppressed non-T cell differentiation potentials.
Function of Bcl11b in mature T cells
Bcl11b plays a critical role in DP thymocytes by controlling positive selection of both CD4 and CD8 lineages. Bcl11b-deficient DP thymocytes are prone to spontaneous apoptosis, which is possibly due to impaired proximal TCR signaling and attenuated extracellular signal-regulated kinase phosphorylation and calcium flux that are required for initiation of positive selection . In mice CD4+ T cells, following activation through TCR, down-regulation of endogenous human BCL11B reduces IL-2 expression, whereas overexpression of BCL11B augments IL-2 expression . Further studies indicate BCL11B promotes IL2 expression by binding and activating the IL2 promoter through the US1 site and by enhancing NFKB1 (NK-kappa-B) activity . For the CD8+ T cells, Bcl11b is essential in the antigen-specific clonal expansion and cytolytic effector function . Loss of Bcl11b can also induce mature T cells, like DP and CD8+, to differentiate into ITNK . It has been suggested that Bcl11b represses gene expression program that are associated with mature CD4+ and CD8+ thymocytes, including Zbtb7b (Th-POK) and Runx3 that are important for the development of mature CD4+ and CD8+ T cells, respectively [135–137].
It is worth mention that Bcl11b haploinsufficiency is demonstrated in thymocyte development. Compared to wild type mice, heterozygous Bcl11b mutant mice have fewer thymocytes. These Bcl11b+/- thymocytes are much more prone to lymphomagenesis . Moreover, in γ-irradiated mice, Bcl11b heterozygosity promotes clonal expansion and differentiation arrest of thymocytes . Nevertheless, it was puzzling that no tumour development has ever been reported in mice transplanted with Bcl11b homozygous knockout progenitors from fetal live.
Reprogramming from T cells to NK-like cells
Using Bcl11b conditional knockout mice, it was demonstrated that Bcl11b is important for both early and mature T cell development. Positively regulated by Notch signaling, Bcl11b suppresses key NK genes expression and promotes the expression of cocktail of T cell-associated transcription factors in T cells. Thus, acute loss of Bcl11b makes early T cells lose their T cell potential and differentiate to NK cells. In committed DN3 thymocytes, Bcl11b is essential for the maintenance of T cell identity, since every single survival Bcl11b deficient DN3 T cell abandons its T cell development program and subsequently reprograms to ITNK. Similarly, mature T cells transdifferentiate to ITNK upon loss of Bcl11b. In a word, Bcl11b is indispensable for T cells at all developmental stages to sustain T cell development and maintain T cell identity, while suppress NK cell program (Figure 5) .
Bcl11b in other tissues
Besides the immune system, Bcl11b is also required in skin, neuron and tooth development [18, 129, 140]. Bcl11b is highly expressed in mouse skin during embryogenesis. In the developing epidermis at late stage of fetal development and in the adult skin, Bcl11b expression decreases and becomes restricted to the proliferating cells of the basal cell layer [17, 128, 129, 140]. Further analysis indicates that a subset of skin stem cells may express Bcl11b [140, 141]. Analysis of mice with gremline deletion of Bcl11b shows that Bcl11b is required in skin during development, particularly in keratinocyte proliferation and late differentiation events [140–142]. Similarly, BCL11B is expressed in human epidermis, and is linked to disease progression and/or maintenance in atopic dermatitis and allergic contact dermatitis patients .
In the nerve system, Bcl11b is essential for the development of corticospinal motor neurons axonal projections to the spinal cord in vivo , and is also indispensable for striatal medium spiny neurons differentiation, striatal patch development, and the establishment of the cellular architecture of the striatum . In humans, BCL11B expression is maintained at high levels in normal adult striatum but significantly decreased in Huntington disease (HD) cells, because mutant Huntington striatal neurons is sensitive to over-expression of BCL11B , suggesting that sequestration and/or decreased BCL11B expression is responsible for the deregulation of striatal gene expression and the specificity of pathology that are observed in HD.
Bcl11b also participates in the regulation of epithelial cell differentiation during tooth morphogenesis and is highly expressed in ectodermic components of the developing tooth. Bcl11b-deficient mice show multiple defects at the bell stage and have abnormal incisors and molars . In addition Bcl11b plays multiple roles in modulating cell migration, cell proliferation and hair follicle stem cell maintenance during cutaneous wounding .
Partners and downstream genes of Bcl11b
Bcl11b, also termed Ctip2 (COUP-TF interacting protein), was independently isolated for its ability to interact with all members of the chicken ovalbumin upstream promoter transcription factor (COUP-TF) subfamily of orphan nuclear receptors . COUP-TFs usually mediate transcriptional repression by recruiting nuclear receptor co-repressor (NCoR) and/or silencing mediator for retinoid and thyroid hormone receptor (SMRT) to the template. COUP-TF family members play important roles in pattern formation in the developing nervous systems of xenopus and drosophila [146–148]. As a COUP-TF-interacting protein, Bcl11b mediates transcriptional repression when tethered to a promoter by interacting with a DNA binding protein, such as ARP1, which is a member of COUP-TF subfamily of orphan nuclear receptors . BCL11B also interacts with two metastasis associated proteins MTA1 and MTA2 directly . In HEK293 cells, BCL11B recruits sirtuin 1 (SIRT1) that is a trichostatin-insensitive, nicotinamide-sensitive class III histone deacetylase to the promoter region of a reporter gene template [114, 149]. In addition, BCL11B recruits histone deacetylase 1 (HDAC1) and HDAC2 to promote local histone H3 deacetylation at the HIV-1 promoter region .
To regulates cell cycle. Bcl11b cooperates with SUV39H1 and histone methylase to silence Cdkn1a (p21) . Cdkn1a (p21) is a major cell cycle regulator which can respond to DNA damage senescence and tumor suppression . In addition, Bcl11b can also repress another cyclin-dependent kinase inhibitor, Cdkn1c (p57KIP2) , which is able to associate with and inhibit the catalytic activity of a number of cylin-cdk complexes . A recent study proposes that the up-stream regulator of Bcl11b is TCF-1 which is activated by Notch1 signals .
The Immune system is the central defender against outside pathogen and neoplasm. The importance of human immune system cannot be too much investigated. So far, millions of patients have been saved for the newly found drugs which are screened or designed by the mounting information about immunological response and tumourgenesis. However, progresses are impeded by in vivo study of human immunology and ethic constrains. Since the genome and their the immune system between human and mouse is similar, making mouse the most widely used model in elucidating key processes of the immune system and in revealing molecular mechanisms of immunological diseases [36, 37]. Based on gene manipulation and humanize mice, the mask of many genes related to immune and hematopoietic system, including Bcl11b, Gata3 and TCF1, are unveiled. So far, programs of the whole genome knockout mice, like the Knockout Mouse Project (KOMP) and the European Conditional Mouse Mutagenesis Program (EUCOMM), have generated thousands of conventional and conditional knockout mouse strains and are distributing them worldwide . Furthermore, with the development of zinc nuclease fingers and TALENs technologies, it is easier to generate gene knockout and knock-in mice. Thus, we expect, in the coming years, the genome engineering technologies and conditional knockout mouse strains will be available for more labs and researchers, so that functions of more transcription factors will be elucidated in T cell development.
Mouse Genetics, Concepts and Applications. USA: Oxford University Press; 1995.
Imada K: Immunodeficient mouse models of lymphoid tumors. Int J Hematol 2003, 77: 336–341.
Zhou P, Hohm S, Capoccia B, Wirthlin L, Hess D, Link D, Nolta J: Immunodeficient mouse models to study human stem cell-mediated tissue repair. Methods Mol Biol 2008, 430: 213–225.
Brehm MA, Cuthbert A, Yang C, Miller DM, DiIorio P, Laning J, Burzenski L, Gott B, Foreman O, Kavirayani A, et al.: Parameters for establishing humanized mouse models to study human immunity: analysis of human hematopoietic stem cell engraftment in three immunodeficient strains of mice bearing the IL2rgamma(null) mutation. Clin Immunol 2010, 135: 84–98.
Rothenberg EV: Transcriptional drivers of the T-cell lineage program. Curr Opin Immunol 2012, 24: 132–138.
Li P, Burke S, Wang J, Chen X, Ortiz M, Lee SC, Lu D, Campos L, Goulding D, Ng BL, et al.: Reprogramming of T cells to natural killer-like cells upon Bcl11b deletion. Science 2010, 329: 85–89.
Li L, Leid M, Rothenberg EV: An early T cell lineage commitment checkpoint dependent on the transcription factor Bcl11b. Science 2010, 329: 89–93.
Ikawa T, Hirose S, Masuda K, Kakugawa K, Satoh R, Shibano-Satoh A, Kominami R, Katsura Y, Kawamoto H: An essential developmental checkpoint for production of the T cell lineage. Science 2010, 329: 93–96.
Brinster RL, Chen HY, Trumbauer M, Senear AW, Warren R, Palmiter RD: Somatic Expression of Herpes Thymidine Kinase in Mice Following Injection of a Fusion Gene into Eggs. Cell 1981, 27: 223–231.
Wagner EF, Stewart TA, Mintz B: The Human Beta-Globin Gene and a Functional Viral Thymidine Kinase Gene in Developing Mice. P Natl Acad Sci-Biol 1981, 78: 5016–5020.
Recchia A, Mavilio F: Site-specific integration by the adeno-associated virus rep protein. Current gene therapy 2011, 11: 399–405.
Ivics Z, Izsvak Z: Nonviral gene delivery with the sleeping beauty transposon system. Human gene therapy 2011, 22: 1043–1051.
Chen YT, Furushima K, Hou PS, Ku AT, Deng JM, Jang CW, Fang H, Adams HP, Kuo ML, Ho HN, et al.: PiggyBac transposon-mediated, reversible gene transfer in human embryonic stem cells. Stem cells and development 2010, 19: 763–771.
Wilson MH, Coates CJ, George AL Jr: PiggyBac transposon-mediated gene transfer in human cells. Molecular therapy: the journal of the American Society of Gene Therapy 2007, 15: 139–145.
Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen R, Cowling R, Wang W, Liu P, Gertsenstein M, et al.: piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 2009, 458: 766–770.
Hickman-Davis JM, Davis IC: Transgenic mice. Paediatr Respir Rev 2006, 7: 49–53.
Antoch MP, Song EJ, Chang AM, Vitaterna MH, Zhao Y, Wilsbacher LD, Sangoram AM, King DP, Pinto LH, Takahashi JS: Functional identification of the mouse circadian Clock gene by transgenic BAC rescue. Cell 1997, 89: 655–667.
Kleinjan DA, Van Heyningen V: Long-range control of gene expression: emerging mechanisms and disruption in disease. Am J Hum Genet 2005, 76: 8–32.
Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse embryos. Nature 1981, 292: 154–156.
Martin GR: Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A 1981, 78: 7634–7638.
Bradley A, Evans M, Kaufman MH, Robertson E: Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature 1984, 309: 255–256.
Robertson E, Bradley A, Kuehn M, Evans M: Germ-Line Transmission of Genes Introduced into Cultured Pluripotential Cells by Retroviral Vector. Nature 1986, 323: 445–448.
Capecchi MR: Altering the Genome by Homologous Recombination. Science 1989, 244: 1288–1292.
Koller BH, Smithies O: Inactivating the Beta-2-Microglobulin Locus in Mouse Embryonic Stem-Cells by Homologous Recombination. P Natl Acad Sci USA 1989, 86: 8932–8935.
Koller BH, Marrack P, Kappler JW, Smithies O: Normal development of mice deficient in beta 2M, MHC class I proteins, and CD8+ T cells. Science 1990, 248: 1227–1230.
Zijlstra M, Bix M, Simister NE, Loring JM, Raulet DH, Jaenisch R: Beta 2-microglobulin deficient mice lack CD4–8+ cytolytic T cells. Nature 1990, 344: 742–746.
Guan CM, Ye C, Yang XM, Gao JG: A Review of Current Large-Scale Mouse Knockout Efforts. Genesis 2010, 48: 73–85.
Hall B, Limaye A, Kulkarni AB: Overview: generation of gene knockout mice. In Current protocols in cell biology Edited by: Bonifacino JS. 2009. Chapter 19:Unit 19 12 19 12 11–17
Copp AJ: Death before Birth - Clues from Gene Knockouts and Mutations. Trends Genet 1995, 11: 87–93.
Betz UA, Vosshenrich CA, Rajewsky K, Muller W: Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination. Current biology: CB 1996, 6: 1307–1316.
Rajewsky K, Gu H, Kuhn R, Betz UA, Muller W, Roes J, Schwenk F: Conditional gene targeting. J Clin Investig 1996, 98: 600–603.
Radtke F, Wilson A, Stark G, Bauer M, Van Meerwijk J, MacDonald HR, Aguet M: Deficient T cell fate specification in mice with an induced inactivation of Notch1. Immunity 1999, 10: 547–558.
Williams R, Lendahl U, Lardelli M: Complementary and combinatorial patterns of Notch gene family expression during early mouse development. Mech Dev 1995, 53: 357–368.
Pai SY, Truitt ML, Ting CN, Leiden JM, Glimcher LH, Ho IC: Critical roles for transcription factor GATA-3 in thymocyte development. Immunity 2003, 19: 863–875.
Wakabayashi Y, Watanabe H, Inoue J, Takeda N, Sakata J, Mishima Y, Hitomi J, Yamamoto T, Utsuyama M, Niwa O, et al.: Bcl11b is required for differentiation and survival of alphabeta T lymphocytes. Nat Immunol 2003, 4: 533–539.
Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, et al.: Initial sequencing and analysis of the human genome. Nature 2001, 409: 860–921.
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, Smith HO, Yandell M, Evans CA, Holt RA, et al.: The sequence of the human genome. Science 2001, 291: 1304–1351.
Drake AC, Chen Q, Chen J: Engineering humanized mice for improved hematopoietic reconstitution. Cell Mol Immunol 2012, 9: 215–224.
Notta F, Doulatov S, Laurenti E, Poeppl A, Jurisica I, Dick JE: Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment. Science 2011, 333: 218–221.
Bosma GC, Custer RP, Bosma MJ: A severe combined immunodeficiency mutation in the mouse. Nature 1983, 301: 527–530.
Leblond V, Autran B, Cesbron JY: The SCID mouse mutant: Definition and potential use as a model for immune and hematological disorders. Hematol Cell Ther 1997, 39: 213–221.
Macchiarini F, Manz MG, Palucka AK, Shultz LD: Humanized mice: are we there yet? The Journal of experimental medicine 2005, 202: 1307–1311.
Shultz LD, Schweitzer PA, Christianson SW, Gott B, Schweitzer IB, Tennent B, McKenna S, Mobraaten L, Rajan TV, Greiner DL, et al.: Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice. J Immunol 1995, 154: 180–191.
Kataoka S, Satoh J, Fujiya H, Toyota T, Suzuki R, Itoh K, Kumagai K: Immunologic aspects of the nonobese diabetic (NOD) mouse Abnormalities of cellular immunity. Diabetes 1983, 32: 247–253.
Sugamura K, Asao H, Kondo M, Tanaka N, Ishii N, Ohbo K, Nakamura M, Takeshita T: The interleukin-2 receptor gamma chain: its role in the multiple cytokine receptor complexes and T cell development in XSCID. Annu Rev Immunol 1996, 14: 179–205.
Cao X, Shores EW, Hu-Li J, Anver MR, Kelsall BL, Russell SM, Drago J, Noguchi M, Grinberg A, Bloom ET, et al.: Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain. Immunity 1995, 2: 223–238.
Shultz LD, Lyons BL, Burzenski LM, Gott B, Chen X, Chaleff S, Kotb M, Gillies SD, King M, Mangada J, et al.: Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells. J Immunol 2005, 174: 6477–6489.
Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, et al.: NOD/SCID/gamma(c)(null) mouse: an excellent recipient mouse model for engraftment of human cells. Blood 2002, 100: 3175–3182.
Shultz LD, Ishikawa F, Greiner DL: Humanized mice in translational biomedical research. Nat Rev Immunol 2007, 7: 118–130.
Harrison PR: Analysis of erythropoeisis at the molecular level. Nature 1976, 262: 353–356.
Rossi L, Lin Kuanyin K, Boles Nathan C, Yang L, King Katherine Y, Jeong M, Mayle A, Goodell Margaret A: Less Is More: Unveiling the Functional Core of Hematopoietic Stem Cells through Knockout Mice. Cell Stem Cell 2012, 11: 302–317.
Godin I, Cumano A: The hare and the tortoise: an embryonic haematopoietic race. Nat Rev Immunol 2002, 2: 593–604.
Morrison SJ, Weissman IL: The Long-Term Repopulating Subset of Hematopoietic Stem-Cells Is Deterministic and Isolatable by Phenotype. Immunity 1994, 1: 661–673.
Akashi K, Kondo M, Cheshier S, Shizuru J, Gandy K, Domen J, Mebius R, Traver D, Weissman IL: Lymphoid development from stem cells and the common lymphocyte progenitors. Cold Spring Harbor symposia on quantitative biology 1999, 64: 1–12.
Iwasaki H, Akashi K: Myeloid lineage commitment from the hematopoietic stem cell. Immunity 2007, 26: 726–740.
Bell JJ, Bhandoola A: The earliest thymic progenitors for T cells possess myeloid lineage potential. Nature 2008, 452: 764–767.
Wada H, Masuda K, Satoh R, Kakugawa K, Ikawa T, Katsura Y, Kawamoto H: Adult T-cell progenitors retain myeloid potential. Nature 2008, 452: 768-U710.
Graf T, Enver T: Forcing cells to change lineages. Nature 2009, 462: 587–594.
Schutte J, Moignard V, Gottgens B: Establishing the stem cell state: insights from regulatory network analysis of blood stem cell development. Wiley interdisciplinary reviews Systems biology and medicine 2012,4(3):285–295.
Xie HF, Ye M, Feng R, Graf T: Stepwise reprogramming of B cells into macrophages. Cell 2004, 117: 663–676.
Rolink AG, Schaniel C, Bruno L, Melchers F: In vitro and in vivo plasticity of Pax5-deficient pre-B I cells. Immunol Lett 2002, 82: 35–40.
Koch G: The Avian Immune-System. Tijdschr Diergeneesk 1991, 116: 728–734.
David-Fung ES, Yui MA, Morales M, Wang H, Taghon T, Diamond RA, Rothenberg EV: Progression of regulatory gene expression states in fetal and adult pro-T-cell development. Immunol Rev 2006, 209: 212–236.
Dudley EC, Petrie HT, Shah LM, Owen MJ, Hayday AC: T-Cell Receptor-Beta Chain Gene Rearrangement and Selection during Thymocyte Development in Adult Mice. Immunity 1994, 1: 83–93.
Fevrier M, Dorgham K, Rebollo A: CD4+ T cell depletion in human immunodeficiency virus (HIV) infection: role of apoptosis. Viruses 2011, 3: 586–612.
Trono D, Van Lint C, Rouzioux C, Verdin E, Barre-Sinoussi F, Chun TW, Chomont N: HIV Persistence and the Prospect of Long-Term Drug-Free Remissions for HIV-Infected Individuals. Science 2010, 329: 174–180.
Ciofani M, Zuniga-Pflucker JC: The thymus as an inductive site for T lymphopoiesis. Annu Rev Cell Dev Bi 2007, 23: 463–493.
Zhang JA, Mortazavi A, Williams BA, Wold BJ, Rothenberg EV: Dynamic transformations of genome-wide epigenetic marking and transcriptional control establish T cell identity. Cell 2012, 149: 467–482.
Adolfsson J, Mansson R, Buza-Vidas N, Hultquist A, Liuba K, Jensen CT, Bryder D, Yang LP, Borge OJ, Thoren LAM, et al.: Identification of Flt3(+) lympho-myeloid stem cells lacking erythro-megakaryocytic potential: A revised road map for adult blood lineage commitment. Cell 2005, 121: 295–306.
Anderson G, Moore NC, Owen JJT, Jenkinson EJ: Cellular interactions in thymocyte development. Annu Rev Immunol 1996, 14: 73–99.
Godfrey DI, Kennedy J, Suda T, Zlotnik A: A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression. J Immunol 1993, 150: 4244–4252.
Luc S, Luis TC, Boukarabila H, Macaulay IC, Buza-Vidas N, Bouriez-Jones T, Lutteropp M, Woll PS, Loughran SJ, Mead AJ, et al.: The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential. Nat Immunol 2012, 13: 412–419.
Naito T, Tanaka H, Naoe Y, Taniuchi I: Transcriptional control of T-cell development. Int Immunol 2011, 23: 661–668.
Hosoya T, Kuroha T, Moriguchi T, Cummings D, Maillard I, Lim KC, Engel JD: GATA-3 is required for early T lineage progenitor development. Journal of Experimental Medicine 2009, 206: 2987–3000.
Sambandam A, Maillard I, Zediak VP, Xu L, Gerstein RM, Aster JC, Pear WS, Bhandoola A: Notch signaling controls the generation and differentiation of early T lineage progenitors. Nat Immunol 2005, 6: 663–670.
Weber BN, Chi AW, Chavez A, Yashiro-Ohtani Y, Yang Q, Shestova O, Bhandoola A: A critical role for TCF-1 in T-lineage specification and differentiation. Nature 2011, 476: 63–68.
Rothenberg EV, Taghon T: Molecular genetics of T cell development. Annu Rev Immunol 2005, 23: 601–649.
Rothenberg EV, Moore JE, Yui MA: Launching the T-cell-lineage developmental programme. Nat Rev Immunol 2008, 8: 9–21.
Jones ME, Zhuang Y: Regulation of V(D)J Recombination by E-Protein Transcription Factors. V(D)J Recombination 2009, 650: 148–156.
Jones ME, Zhuang YA: Stage-specific functions of E-proteins at the beta-selection and T-cell receptor checkpoints during thymocyte development. Immunol Res 2011, 49: 202–215.
Nikolic-Zugic J, Moore MW: T cell receptor expression on immature thymocytes with in vivo and in vitro precursor potential. Eur J Immunol 1989, 19: 1957–1960.
Vukmanovic S, Grandea AG, Faas SJ, Knowles BB, Bevan MJ: Positive Selection of Lymphocytes-T Induced by Intrathymic Injection of a Thymic Epithelial-Cell Line. Nature 1992, 359: 729–732.
Castro JE, Listman JA, Jacobson BA, Wang Y, Lopez PA, Ju S, Finn PW, Perkins DL: Fas modulation of apoptosis during negative selection of thymocytes. Immunity 1996, 5: 617–627.
Takada K, Jameson SC: Naive T cell homeostasis: from awareness of space to a sense of place. Nat Rev Immunol 2009, 9: 823–832.
Anderson G, Jenkinson WE, Jones T, Parnell SM, Kinsella FA, White AJ, Pongrac'z JE, Rossi SW, Jenkinson EJ: Establishment and functioning of intrathymic microenvironments. Immunol Rev 2006, 209: 10–27.
Jones-Mason ME, Zhao X, Kappes D, Lasorella A, Iavarone A, Zhuang Y: E Protein Transcription Factors Are Required for the Development of CD4(+) Lineage T Cells. Immunity 2012, 36: 348–361.
Herberman RB, Nunn ME, Holden HT, Lavrin DH: Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. International journal of cancer Journal international du cancer 1975, 16: 230–239.
Kiessling R, Klein E, Pross H, Wigzell H: "Natural" killer cells in the mouse II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur J Immunol 1975, 5: 117–121.
Kiessling R, Haller O, Fenyo EM, Steinitz M, Klein G: Mouse Natural Killer (Nk) Cell Activity against Human Cell Lines Is Not Influenced by Super-Infection of Target-Cell with Xenotropic Murine C-Type Virus. Int J Cancer 1978, 21: 460–465.
Oldham RK: Natural-Killer Cells - Artifact to Reality - an Odyssey in Biology. Cancer Metast Rev 1983, 2: 323–336.
Guerra N, Tan YX, Joncker NT, Choy A, Gallardo F, Xiong N, Knoblaugh S, Cado D, Greenberg NM, Raulet DH: NKG2D-deficient mice are defective in tumor surveillance in models of spontaneous malignancy (vol 28, pg 571, 2008). Immunity 2008, 28: 723–723.
Michie AM, Carlyle JR, Schmitt TM, Ljutic B, Cho SK, Fong QY, Zuniga-Pflucker JC: Clonal characterization of a bipotent T cell and NK cell progenitor in the mouse fetal thymus. J Immunol 2000, 164: 1730–1733.
Di Santo JP, Vosshenrich CA: Bone marrow versus thymic pathways of natural killer cell development. Immunol Rev 2006, 214: 35–46.
Puzanov IJ, Bennett M, Kumar V: IL-15 can substitute for the-marrow microenvironment in the differentiation of natural killer cells. J Immunol 1996, 157: 4282–4285.
Kim S, Iizuka K, Kang HSP, Dokun A, French AR, Greco S, Yokoyama WM: In vivo developmental stages in murine natural killer cell maturation. Nat Immunol 2002, 3: 523–528.
Takeda K, Cretney E, Hayakawa Y, Ota T, Akiba H, Ogasawara K, Yagita H, Kinoshita K, Okumura K, Smyth MJ: TRAIL identifies immature natural killer cells in newborn mice and adult mouse liver. Blood 2005, 105: 2082–2089.
Yoshizawa K, Nakajima S, Notake T, Miyagawa S, Hida S, Taki S: IL-15-high-responder developing NK cells bearing Ly49 receptors in IL-15-/- mice. J Immunol 2011, 187: 5162–5169.
Colucci F, Caligiuri MA, Di Santo JP: What does it take to make a natural killer? Nat Rev Immunol 2003, 3: 413–425.
Vosshenrich CAJ, Garcia-Ojeda ME, Samson-Villeger SI, Pasqualetto V, Enault L, Goff ORL, Corcuff E, Guy-Grand D, Rocha B, Cumano A, et al.: A thymic pathway of mouse natural killer cell development characterized by expression of GATA-3 and CD127 (vol 7, pg 1217, 2006). Nat Immunol 2006, 7: 1343–1343.
Hidalgo L, Martinez VG, Valencia J, Hernandez-Lopez C, Vazquez MN, Nunez JR, Zapata AG, Sacedon R, Varas A, Vicente A: Expression of BMPRIA on human thymic NK cell precursors: role of BMP signaling in intrathymic NK cell development. Blood 2012, 119: 1861–1871.
Narni-Mancinelli E, Jaeger BN, Bernat C, Fenis A, Kung S, De Gassart A, Mahmood S, Gut M, Heath SC, Estelle J, et al.: Tuning of natural killer cell reactivity by NKp46 and Helios calibrates T cell responses. Science 2012, 335: 344–348.
Liu P, Li P, Burke S: Critical roles of Bcl11b in T-cell development and maintenance of T-cell identity. Immunol Rev 2010, 238: 138–149.
Satterwhite E, Sonoki T, Willis TG, Harder L, Nowak R, Arriola EL, Liu H, Price HP, Gesk S, Steinemann D, et al.: The BCL11 gene family: involvement of BCL11A in lymphoid malignancies. Blood 2001, 98: 3413–3420.
Nakamura T, Largaespada DA, Shaughnessy JD Jr, Jenkins NA, Copeland NG: Cooperative activation of Hoxa and Pbx1-related genes in murine myeloid leukaemias. Nat Genet 1996, 12: 149–153.
Liu PT, Keller JR, Ortiz M, Tessarollo L, Rachel RA, Nakamura T, Jenkins NA, Copeland NG: Bcl11a is essential for normal lymphoid development. Nat Immunol 2003, 4: 525–532.
Tydell CC, David-Fung ES, Moore JE, Rowen L, Taghon T, Rothenberg EV: Molecular dissection of prethymic progenitor entry into the T lymphocyte developmental pathway. J Immunol 2007, 179: 421–438.
Menzel S, Garner C, Gut I, Matsuda F, Yamaguchi M, Heath S, Foglio M, Zelenika D, Boland A, Rooks H, et al.: A QTL influencing F cell production maps to a gene encoding a zinc-finger protein on chromosome 2p15. Nat Genet 2007, 39: 1197–1199.
Uda M, Galanello R, Sanna S, Lettre G, Sankaran VG, Chen WM, Usala G, Busonero F, Maschio A, Albai G, et al.: Genome-wide association study shows BCL11A associated with persistent fetal hemoglobin and Amelioration of the phenotype of beta-thalassemia. P Natl Acad Sci USA 2008, 105: 1620–1625.
Sankaran VG, Xu J, Ragoczy T, Ippolito GC, Walkley CR, Maika SD, Fujiwara Y, Ito M, Groudine M, Bender MA, et al.: Developmental and species-divergent globin switching are driven by BCL11A. Nature 2009, 460: 1093-U1050.
Simonis-Bik AM, Nijpels G, Van Haeften TW, Houwing-Duistermaat JJ, Boomsma DI, Reiling E, Van Hove EC, Diamant M, Kramer MH, Heine RJ, et al.: Gene variants in the novel type 2 diabetes loci CDC123/CAMK1D, THADA, ADAMTS9, BCL11A, and MTNR1B affect different aspects of pancreatic beta-cell function. Diabetes 2010, 59: 293–301.
John A, Brylka H, Wiegreffe C, Simon R, Liu P, Jüttner R, Crenshaw EB III, Luyten FP, Jenkins NA, Copeland NG: Bcl11a is required for neuronal morphogenesis and sensory circuit formation in dorsal spinal cord development. Development 2012,139(10):1831–41. http://www.ncbi.nlm.nih.gov/pubmed?term=Bcl11a%20is%20required%20for%20neuronal%20morphogenesis%20and%20sensory%20circuit%20formation%20in%20dorsal%20spinal%20cord%20development
Gutierrez A, Kentsis A, Sanda T, Holmfeldt L, Chen SC, Zhang JH, Protopopov A, Chin L, Dahlberg SE, Neuberg DS, et al.: The BCL11B tumor suppressor is mutated across the major molecular subtypes of T-cell acute lymphoblastic leukemia. Blood 2011, 118: 4169–4173.
Marban C, Suzanne S, Dequiedt F, De Walque S, Redel L, Van Lint C, Aunis D, Rohr O: Recruitment of chromatin-modifying enzymes by CTIP2 promotes HIV-1 transcriptional silencing. EMBO J 2007, 26: 412–423.
Senawong T, Peterson VJ, Avram D, Shepherd DM, Frye RA, Minucci S, Leid M: Involvement of the histone deacetylase SIRT1 in chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting protein 2-mediated transcriptional repression. J Biol Chem 2003, 278: 43041–43050.
Cismasiu VB, Adamo K, Gecewicz J, Duque J, Lin QS, Avram D: BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter. Oncogene 2005, 24: 6753–6764.
Topark-Ngarm A, Golonzhka O, Peterson VJ, Barrett B, Martinez B, Crofoot K, Filtz TM, Leid M: CTIP2 associates with the NuRD complex on the promoter of p57KIP2, a newly identified CTIP2 target gene. J Biol Chem 2006, 281: 32272–32283.
Wakabayashi Y, Inoue J, Takahashi Y, Matsuki A, Kosugi-Okano H, Shinbo T, Mishima Y, Niwa O, Kominami R: Homozygous deletions and point mutations of the Rit1/Bcl11b gene in gamma-ray induced mouse thymic lymphomas. Biochem Biophys Res Commun 2003, 301: 598–603.
Matsumoto Y, Kosugi S, Shinbo T, Chou D, Ohashi M, Wakabayashi Y, Sakai K, Okumoto M, Mori N, Aizawa S, et al.: Allelic loss analysis of gamma-ray-induced mouse thymic lymphomas: two candidate tumor suppressor gene loci on chromosomes 12 and 16. Oncogene 1998, 16: 2747–2754.
Bernard OA, Busson-LeConiat M, Ballerini P, Mauchauffe M, Della Valle V, Monni R, Nguyen Khac F, Mercher T, Penard-Lacronique V, Pasturaud P, et al.: A new recurrent and specific cryptic translocation, t(5;14)(q35;q32), is associated with expression of the Hox11L2 gene in T acute lymphoblastic leukemia. Leukemia: official journal of the Leukemia Society of America, Leukemia Research Fund, UK 2001, 15: 1495–1504.
MacLeod RA, Nagel S, Kaufmann M, Janssen JW, Drexler HG: Activation of HOX11L2 by juxtaposition with 3'-BCL11B in an acute lymphoblastic leukemia cell line (HPB-ALL) with t(5;14)(q35;q32.2). Genes, chromosomes & cancer 2003, 37: 84–91.
Przybylski GK, Dik WA, Wanzeck J, Grabarczyk P, Majunke S, Martin-Subero JI, Siebert R, Dolken G, Ludwig WD, Verhaaf B, et al.: Disruption of the BCL11B gene through inv(14)(q11.2q32.31) results in the expression of BCL11B-TRDC fusion transcripts and is associated with the absence of wild-type BCL11B transcripts in T-ALL. Leukemia: official journal of the Leukemia Society of America, Leukemia Research Fund, UK 2005, 19: 201–208.
Huang X, Chen S, Chen SH, Yang LJ, Shen Q, Grabarczyk P, Przybylski GK, Schmidt CA, Li YQ: Inhibition of BCL11B Expression Leads to Apoptosis of Malignant T Cells but Not CD34(+). Blood 2010, 116: 1539–1539.
Karanam NK, Grabarczyk P, Hammer E, Scharf C, Venz S, Gesell-Salazar M, Barthlen W, Przybylski GK, Schmidt CA, Volker U: Proteome analysis reveals new mechanisms of Bcl11b-loss driven apoptosis. J Proteome Res 2010, 9: 3799–3811.
Huang X, Chen S, Shen Q, Yang L, Grabarczyk P, Przybylski GK, Schmidt CA, Li Y: Down regulation of BCL11B expression inhibits proliferation and induces apoptosis in malignant T cells by BCL11B-935-siRNA. Hematology 2011, 16: 236–242.
Guo P, Hirano M, Herrin BR, Li J, Yu C, Sadlonova A, Cooper MD: Dual nature of the adaptive immune system in lampreys. Nature 2009, 459: 796–801.
Bajoghli B, Aghaallaei N, Hess I, Rode I, Netuschil N, Tay BH, Venkatesh B, Yu JK, Kaltenbach SL, Holland ND, et al.: Evolution of genetic networks underlying the emergence of thymopoiesis in vertebrates. Cell 2009, 138: 186–197.
Chen S, Huang X, Yang L, Shen Q, Zheng H, Li B, Grabarczyk P, Przybylski GK, Schmidt CA, Li Y: The role of BCL11B in regulating the proliferation of human naive T cells. Hum Immunol 2012,73(5):456–64. [http://www.ncbi.nlm.nih.gov/pubmed?term=The%20role%20of%20BCL11B%20in%20regulating%20the%20proliferation%20of%20human%20naive%20T%20cells]
Arlotta P, Molyneaux BJ, Chen J, Inoue J, Kominami R, Macklis JD: Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Neuron 2005, 45: 207–221.
Arlotta P, Molyneaux BJ, Jabaudon D, Yoshida Y, Macklis JD: Ctip2 controls the differentiation of medium spiny neurons and the establishment of the cellular architecture of the striatum. J Neurosci 2008, 28: 622–632.
Okazuka K, Wakabayashi Y, Kashihara M, Inoue J, Sato T, Yokoyama M, Aizawa S, Aizawa Y, Mishima Y, Kominami R: p53 prevents maturation of T cell development to the immature CD4(-)CD8(+) stage in Bcl11b(-/-) mice. Biochem Bioph Res Co 2005, 328: 545–549.
Albu DI, Feng DY, Bhattacharya D, Jenkins NA, Copeland NG, Liu PT, Avram D: BCL11B is required for positive selection and survival of double-positive thymocytes. J Exp Med 2007, 204: 3003–3015.
Cismasiu VB, Ghanta S, Duque J, Albu DI, Chen HM, Kasturi R, Avram D: BCL11B participates in the activation of IL2 gene expression in CD4+ T lymphocytes. Blood 2006, 108: 2695–2702.
Cismasiu VB, Duque J, Paskaleva E, Califano D, Ghanta S, Young HA, Avram D: BCL11B enhances TCR/CD28-triggered NF-kappaB activation through up-regulation of Cot kinase gene expression in T-lymphocytes. Biochem J 2009, 417: 457–466.
Zhang S, Rozell M, Verma RK, Albu DI, Califano D, VanValkenburgh J, Merchant A, Rangel-Moreno J, Randall TD, Jenkins NA, et al.: Antigen-specific clonal expansion and cytolytic effector function of CD8+ T lymphocytes depend on the transcription factor Bcl11b. The Journal of experimental medicine 2010, 207: 1687–1699.
Dave VP, Allman D, Keefe R, Hardy RR, Kappes DJ: HD mice: a novel mouse mutant with a specific defect in the generation of CD4(+) T cells. Proc Natl Acad Sci U S A 1998, 95: 8187–8192.
Kastner P, Chan S, Vogel WK, Zhang LJ, Topark-Ngarm A, Golonzhka O, Jost B, Le Gras S, Gross MK, Leid M: Bcl11b represses a mature T-cell gene expression program in immature CD4(+)CD8(+) thymocytes. Eur J Immunol 2010, 40: 2143–2154.
Taniuchi I, Osato M, Egawa T, Sunshine MJ, Bae SC, Komori T, Ito Y, Littman DR: Differential requirements for Runx proteins in CD4 repression and epigenetic silencing during T lymphocyte development. Cell 2002, 111: 621–633.
Kamimura K, Ohi H, Kubota T, Okazuka K, Yoshikai Y, Wakabayashi YI, Aoyagi Y, Mishima Y, Kominami R: Haploinsufficiency of Bcl11b for suppression of lymphomagenesis and thymocyte development. Biochem Bioph Res Co 2007, 355: 538–542.
Go R, Hirose S, Morita S, Yamamoto T, Katsuragi Y, Mishima Y, Kominami R: Bcl11b heterozygosity promotes clonal expansion and differentiation arrest of thymocytes in gamma-irradiated mice. Cancer science 2010, 101: 1347–1353.
Golonzhka O, Leid M, Indra G, Indra AK: Expression of COUP-TF-interacting protein 2 (CTIP2) in mouse skin during development and in adulthood. Gene expression patterns: GEP 2007, 7: 754–760.
Golonzhka O, Liang X, Messaddeq N, Bornert JM, Campbell AL, Metzger D, Chambon P, Ganguli-Indra G, Leid M, Indra AK: Dual role of COUP-TF-interacting protein 2 in epidermal homeostasis and permeability barrier formation. J Invest Dermatol 2009, 129: 1459–1470.
Golonzhka O, Metzger D, Bornert JM, Bay BK, Gross MK, Kioussi C, Leid M: Ctip2/Bcl11b controls ameloblast formation during mammalian odontogenesis. Proc Natl Acad Sci U S A 2009, 106: 4278–4283.
Ganguli-Indra G, Liang X, Hyter S, Leid M, Hanifin J, Indra AK: Expression of COUP-TF-interacting protein 2 (CTIP2) in human atopic dermatitis and allergic contact dermatitis skin. Exp Dermatol 2009, 18: 994–996.
Desplats PA, Lambert JR, Thomas EA: Functional roles for the striatal-enriched transcription factor, Bcl11b, in the control of striatal gene expression and transcriptional dysregulation in Huntington's disease. Neurobiol Dis 2008, 31: 298–308.
Liang X, Bhattacharya S, Bajaj G, Guha G, Wang Z, Jang HS, Leid M, Indra AK, Ganguli-Indra G: Delayed cutaneous wound healing and aberrant expression of hair follicle stem cell markers in mice selectively lacking Ctip2 in epidermis. PLoS One 2012, 7: e29999.
Avram D, Fields A, Pretty On Top K, Nevrivy DJ, Ishmael JE, Leid M: Isolation of a novel family of C(2)H(2) zinc finger proteins implicated in transcriptional repression mediated by chicken ovalbumin upstream promoter transcription factor (COUP-TF) orphan nuclear receptors. J Biol Chem 2000, 275: 10315–10322.
Mlodzik M, Hiromi Y, Weber U, Goodman CS, Rubin GM: The Drosophila seven-up gene, a member of the steroid receptor gene superfamily, controls photoreceptor cell fates. Cell 1990, 60: 211–224.
Van der Wees J, Matharu PJ, De Roos K, Destree OH, Godsave SF, Durston AJ, Sweeney GE: Developmental expression and differential regulation by retinoic acid of Xenopus COUP-TF-A and COUP-TF-B. Mech Dev 1996, 54: 173–184.
Senawong T, Peterson VJ, Leid M: BCL11A-dependent recruitment of SIRT1 to a promoter template in mammalian cells results in histone deacetylation and transcriptional repression. Arch Biochem Biophys 2005, 434: 316–325.
Cherrier T, Suzanne S, Redel L, Calao M, Marban C, Samah B, Mukerjee R, Schwartz C, Gras G, Sawaya BE, et al.: p21(WAF1) gene promoter is epigenetically silenced by CTIP2 and SUV39H1. Oncogene 2009, 28: 3380–3389.
Bunz F, Dutriaux A, Lengauer C, Waldman T, Zhou S, Brown JP, Sedivy JM, Kinzler KW, Vogelstein B: Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science 1998, 282: 1497–1501.
Topark-Ngarm A, Golonzhka O, Peterson VJ, Barrett B Jr, Martinez B, Crofoot K, Filtz TM, Leid M: CTIP2 associates with the NuRD complex on the promoter of p57KIP2, a newly identified CTIP2 target gene. J Biol Chem 2006, 281: 32272–32283.
Cunningham JJ, Roussel MF: Cyclin-dependent kinase inhibitors in the development of the central nervous system. Cell growth & differentiation: the molecular biology journal of the American Association for Cancer Research 2001, 12: 387–396.
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, et al.: A conditional knockout resource for the genome-wide study of mouse gene function. Nature 2011, 474: 337–342.
The authors declare that they have no competing interests.
PL (Peng Li) and YX mainly wrote the manuscript and drew the pictures in this manuscript. PL (Pentao Liu) modified this manuscript and gave valuable advices. ZL proof read this manuscript. All authors read and approved the final manuscript.