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Cell Regeneration

Table 3 Culture surface coating requirements and costing for the generation of 1 billion (x10 9 ) hPSCs

From: Defining synthetic surfaces for human pluripotent stem cell culture

Surface

Manufacturer

Coating density source

Cost per cm 2($USD) 1

Cost per 1 trillion cells (5000 cm 2) ($USD)

MatrigelTM

Becton Dickinson

Becton Dickinson handbook

$0.080

$400

Cat No: 354277

GeltrexTM

Invitrogen

Life TechnologiesTM handbook

$0.062

$310

Cat No: A1413302

Recombinant human laminin-511 (whole protein)

BioLamina

[112] 2

$10.7

$53331

Cat No: LN511

Recombinant human vitronectin (truncated protein)

Gibco®

[73]

$0.0413

$205

Cat No: A14701SA

Recombinant human fibronectin

Abcam

[113]

$0.664

$3320

Cat No: AB92798

Corning SynthemaxTM II-SC

Corning Inc.

Corning handbook

$0.205

$1026

Cat No: 3535XX1

StemAdhereTM(E-cadherin fusion protein)

Primorigen Biosciences ® Cat No: S2112

Primorigen handbook

$0.081

$406

  1. Calculations were based on a typical cell density of 2x105 cells/cm2. These calculations do not take into account requirements for media or plasticware or the implementation of cardiomyocyte differentiation protocols. It should also be noted that such protocols are not 100% efficient, so it is likely that additional cells would be required.
  2. 1Prices were obtained from the websites of Australian suppliers of the manufacturers listed and converted from $AUD to $USD on the ninth of April 2013 ($1AUD =1.04$USD).
  3. 2Only concentrations of ECMPs in solution were reported so a volume of 50ul/cm2 was used for calculations, based on recommendations for MatrigelTM coatings (Becton Dickinson).
  4. 3At the time of writing recombinant vitronectin was being promoted in combination with the hPSC media E8 and as such was being sold at low cost.
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