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Figure 1 | Cell Regeneration

Figure 1

From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system

Figure 1

Genome editing via the Cas9/gRNA system in rabbit. (A) Constructs and schematic illustration of the Cas9/gRNA system used in this study. The T7 promoter drives transcription of the gRNA, consisting of a target sequence and a scaffold sequence. NLS: nuclear localization signal. bGH polyA: bovine growth hormone poly-A. (B) Target sequence of the IL2rg, RAG1, RAG2, TIKI1 and ALB genes using the Cas9/gRNA system. gRNA-targeting sequences are underlined and PAM sequences are highlighted in red.

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