One gene (TIKI1)-KO rabbit embryos and newborn rabbits. (A) Sequenced mutations in the TIKI1 gene in injected embryos. Deletions are indicated by dashes and insertions are indicated in blue. (B) Generation of TIKI1 KO rabbits by the Cas9/gRNA system. The zygotes (n = 30) microinjection with the 200 ng/μL of Cas9 mRNA and 20 ng/μL of gRNA for TIKI1 were transferred to 3 recipient mothers, 2 of which gave birth to 5 live kits. (C) Detailed mutations of the TIKI1 gene in the 5 KO founders. The number of founder KO rabbits is shown in the left column. In A and C, the WT sequence is shown at the top with the target sites in underline, and the sizes of the deletions (-) or insertions (+) are shown in the right column.