Table 1 In vitro development of embryos injected with Cas9 mRNA and gRNAs knocking out a single gene
From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system
Gene | gRNA/Cas9 mRNA (ng/μL) | No. of injected zygotes | No. of blastocysts (%) | No. of sequenced blastocysts | No. of modified (%) | No. of biallelic- modified (%) |
|---|---|---|---|---|---|---|
IL2rg | 20/200 | 21 | 16 (76) | 12 | 12 (100) | 12 (100) * |
TIKI1 | 20/200 | 10 | 8 (80) | 8 | 8 (100) | 4 (50) |