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Table 1 In vitro development of embryos injected with Cas9 mRNA and gRNAs knocking out a single gene

From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system

Gene gRNA/Cas9 mRNA (ng/μL) No. of injected zygotes No. of blastocysts (%) No. of sequenced blastocysts No. of modified (%) No. of biallelic- modified (%)
IL2rg 20/200 21 16 (76) 12 12 (100) 12 (100) *
TIKI1 20/200 10 8 (80) 8 8 (100) 4 (50)
  1. *Indicates embryos without detecting WT sequence.