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Table 2 In vitro development of embryos injected with Cas9 mRNA and gRNAs knocking out 3 genes simultaneously

From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system

Gene gRNA/cas9 mRNA (ng/μL) No. of injected zygotes No. of blastocysts (%) No. of sequenced blastocysts No. of modified (%) No. of biallelic-modified (%) No. of 2 gene- modified (%) No. of 3 gene- modified (%) No. of 3 gene biallelic-modified (%)
IL2rg     18 18 (100) 18 (100)*    
RAG1 20/20/20/200 24 20 (83.3) 19 19 (100) 17 (89.5) 18/18 (100) 16/16 (100) 15/16 (93.75)*
RAG2     16 16 (100) 16 (100)    
  1. *indicates embryos without detecting WT sequence.