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Table 2 In vitro development of embryos injected with Cas9 mRNA and gRNAs knocking out 3 genes simultaneously

From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system

Gene

gRNA/cas9 mRNA (ng/μL)

No. of injected zygotes

No. of blastocysts (%)

No. of sequenced blastocysts

No. of modified (%)

No. of biallelic-modified (%)

No. of 2 gene- modified (%)

No. of 3 gene- modified (%)

No. of 3 gene biallelic-modified (%)

IL2rg

   

18

18 (100)

18 (100)*

   

RAG1

20/20/20/200

24

20 (83.3)

19

19 (100)

17 (89.5)

18/18 (100)

16/16 (100)

15/16 (93.75)*

RAG2

   

16

16 (100)

16 (100)

   
  1. *indicates embryos without detecting WT sequence.