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Table 3 In vitro development of embryos injected with Cas9 mRNA and gRNAs knocking out simultaneously 5 genes

From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system

Gene gRNA/cas9 mRNA (ng/μL) No. of injected zygotes No. of blastocysts (%) No. of sequenced blastocysts No. of modified for a single gene (%) No. of biallelic- modified (%) No. of 2 or more genes- modified (%) No. of 3 or more genes- modified (%) No. of 4 or more genes- modified (%) No. of 5 genes- modified (%) No. of 5 genes biallelic-modified (%)
IL2rg     12 11 (91.7) 11 (91.7) *      
RAG1     15 14 (93.3) 12 (80.0)      
RAG2 20/20/20/20/20/200 39 20 (51.3) 14 12 (85.7) 10 (71.4) 11/12 (91.7) 7/12 (58.3) 6/12 (50.0) 4/12 (33.3) 1/12 (8.33)*
TIKI1     14 9 (64.3) 6 (42.9)      
ALB     19 11 (57.9) 5 (26.3)      
  1. *indicates embryos without detecting WT sequence.