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Figure 2 | Cell Regeneration

Figure 2

From: Characterization of a novel cell penetrating peptide derived from human Oct4

Figure 2

Cellular uptake determined by flow cytometry. Cells were incubated with 0, 2.5, 5, 10, 15 μM of FITC-labelled Oct4-PTD (black, solid) or Penetratin (gray, dashed) for 60 min at 37°C, washed with a heparin containing buffer to remove extracellular peptide and analysed for uptake. The results represent the mean fluorescence intensity (MFI) of 3 (15 μM) or 4 (2.5, 5, 10 μM) independent experiments (N = 3-4) with 4 biological replicates per experiment (n = 4). Compared to the untreated cells, both peptides show significant uptake even at the lowest concentration. **p-value ≤ 0.001.

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