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Figure 1 | Cell Regeneration

Figure 1

From: Generation of knockout rabbits using transcription activator-like effector nucleases

Figure 1

Schematic depiction of how ZFNs and TALENs recognize target DNA and induce genome editing. ZFP stands for zinc-finger protein, NLS for nuclear localization signal, N-terminal and C-terminal for amino- and carboxyl-terminal, respectively. DSBs induced by the designer nucleases can be repaired by homology-directed repair or nonhomologous end joining, which can result in knock-ins or knockouts, respectively. FOKI can be substituted by other restriction endonucleases [22].

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