Figure 1From: Generation of knockout rabbits using transcription activator-like effector nucleases Schematic depiction of how ZFNs and TALENs recognize target DNA and induce genome editing. ZFP stands for zinc-finger protein, NLS for nuclear localization signal, N-terminal and C-terminal for amino- and carboxyl-terminal, respectively. DSBs induced by the designer nucleases can be repaired by homology-directed repair or nonhomologous end joining, which can result in knock-ins or knockouts, respectively. FOKI can be substituted by other restriction endonucleases [22].Back to article page