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Fig. 3 | Cell Regeneration

Fig. 3

From: GATA2−/− human ESCs undergo attenuated endothelial to hematopoietic transition and thereafter granulocyte commitment

Fig. 3

Characterization of subtype blood lineages from H1 or H1-GATA2 −/− derived HPCs. a CFU potential cells from H1 or H1-GATA2 −/− were restricted within CD34+CD43+ subpopulations. EC endothelial cells, MC mesenchymal cells. b Characterization of erythrocytes from H1 or H1-GATA2 −/−. From left to right: phase-contrast photographs of BFU and CFU-E, FACS analysis of CD235a and CD71a expression on H1 and H1-GATA2 −/− derived erythrocytes, and cytospin of H1 and H1-GATA2 −/− derived erythrocytes. c Globin analysis of erythrocytes by RT-qPCR. The results showed the mean + SEM of one single experiment with three replicates, representative of three independent experiments. d Analysis of expression of GATA1, GATA2, and GATA3 in H1 or H1-GATA2 −/− derived erythrocytes. The results showed the mean + SEM of one single experiment with three replicates, representative of three independent experiments. e Characterization of myeloid cells from H1 or H1-GATA2 −/−. Left: morphologies of indicated CFU colonies; middle: FACS analysis of indicated markers; right: cytospin photographs of indicated colonies. f FACS analysis of CD86 and CD14 expression in H1 and H1-GATA2 −/− derived myeloid CFU. E erythrocyte, G granulocyte, M macrophage, GM G and M, Mix G, E, and M

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