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Table 1 Differences between BMSCs and ASCs in co-culture with ECs

From: Vascularization mediated by mesenchymal stem cells from bone marrow and adipose tissue: a comparison

  BMSCs ASCs
3D matrix used for co-culture Fibrin [9, 38, 39, 42, 43, 49], polyurethane [37], type I collagen [38], fibrin + type I collagen in different ratios [38] Fibrin [9, 33, 43, 51, 52]
Pericytic marker α-SMA [9, 37, 46], NG2 [37], CD146 [37] α-SMA [9, 50], NG2 [33]
Paracrine factors HGF, TIMP1, TIMP2 [9] HGF, TNF-α [51], PD-ECGF, FGF-2, MMP-9, Ang-2, pentraxin-3 [33], ECM proteins: collagen IV, fibronectin [50]
Altered gene expression FGF-2 ↑, TGFB1 ↑, VEGFA ↑ [42], vWF ↑, CD31 ↑, VE-cadherin ↑, angiopoietin-related protein 4 ↑, CD34 ↑, CD93 ↑, CDH5 ↑ [45], MMPs ↑ [39] CD31 ↑, VE-cadherin ↑, VEGFR-2 ↑, vWF ↑ [33]
Endothelial cell type HUVECs [9, 3739, 43, 45, 46, 48, 49],EPCs [37], ECFCs [42] HUVECs [9, 33, 43, 51], OECs [33, 52]
MMPs important for network formation and ECM degradation Yes (in contrast to fibroblasts) [39, 49] Yes [52], to a lesser extent as plasmin family proteases [51]
  1. BMSCs and ASCs have different effects on ECs and show altered behavior concerning pericytic marker expression, paracrine factors, gene expression, and importance of MMPs for network formation. Furthermore, different matrices as well as different EC types were used to investigate effects of MSCs on ECs