Fig. 5
From: Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish
Inhibition of the N-glycosylation pathway enhances hair cell regeneration. a The N-glycosylation pathway and the inhibition targets of swainsonine and 1-deoxynojirimycin (DNJ). The targets of inhibition for swainsonine and DNJ are indicated by red arrows. b Swainsonine treatment causes a transient increase in hair cell regeneration. There is a significant difference at 2 dpa between the control and 5 μg/ml swainsonine treatment, as well as between the control and 50 μg/ml of swainsonine (p < 0.001 for both). There is no difference between swainsonine at 5 and 50 μg/ml (p = 0.063). c DNJ treatment causes a transient increase in hair cell regeneration. Graphs represent data from the analysis of 10 embryos per data point. Error bars show the s.e.m. There is a significant difference at 2 dpa between the control and 250 μM of DNJ (p = 0.014)