Fig. 2

Fast and high-efficient posterior spinal cord motor neuron differentiation from hSCNPCs. A Schematic illustration of direct spinal motor neurons differentiation from hSCNPCs and hiSCNPCs (derived from H9, DC60-3 and DC87-3). B Immunofluorescent images demonstrating the expression of motor neuron markers HB9, ISL1 of hSCNPCs direct differentiated spinal motor neurons at day 6. Scale bar, 50 μm. C Immunofluorescent images demonstrating the expression of mature motor neuron marker SMI-32 and mature neuronal markers NEUN and MAP2 of hSCNPCs direct differentiated spinal motor neurons at day 18. Scale bar, 50 μm. D Quantification of results shown in Fig. 2B and C. n = 5 independent experiments. Data are represented as mean ± SD. E Immunofluorescent images of neurotransmitter markers ChAT, VAChT, VGLUT, GAD67 and TH of hSCNPCs-derived spinal motor neurons at day 18. Scale bar, 50 μm. F Quantification of results shown in Fig. 2E. n = 5 independent experiments. Data are represented as mean ± SD. G Immunofluorescent staining of HOX family genes HOXB4, HOXC6, HOXC9, HOXD10 and HOXD11 of hSCNPCs-derived spinal motor neurons at day 18. Scale bar, 50 μm. H Quantification of results shown in Fig. 2F. n = 5 independent experiments. Data are represented as mean ± SD. I Principal component analysis of samples from hSCNPCs to spinal motor neurons at each time point shown in Fig. 2A. J 39 HOX genes expression profiles during spinal motor neuron differentiation from hSCNPCs