Fig. 5
From: Deer antler renewal gives insights into mammalian epimorphic regeneration
Longitudinal histology sections of rat leg stumps cut through mid-part of the front leg (Zhang et al., unpublished), which was then left untreated (A and B) or subjected to bioelectric stimulation to the stump periosteum (C) and removal of the interposing layers between the periosteum and the enveloping skin (D). Note that the stump that was stimulated by bioelectricity to the periosteum immediately after amputation showed discrete blue color dots (alcian blue staining), which are the attachment sites for electrodes. Two months after the treatment, the treated stump partially regenerated the lost leg, and the regenerate length is significantly longer than its width. The black line denotes amputation plane