Fig. 2

Identification of a species-specific cell cluster in pig antral epithelium. A Dim plots show the gastric antrum epithelial cells from human, pig, rat, and mouse, respectively. B Dim plots show the expression of F3⁺ cell marker genes, AQP5, CLCA1, F3 and RRAD. C Expression pattern of F3, GS-II and E-cadherin in the antrum of human, pig, rat, and mouse, indicated by immunofluorescence staining (n = 3). Scale bars, 100 μm. D The copy number in 1 μg RNA and the expression of F3 and CLCA1 (n = 3 independent experiments) (left). The FPKM of F3 and CLCA1 in antrum organoids (right). E Function enrichment analysis of the highly expressed genes in F3⁺ cells identified by scRNA-seq