Fig. 1
From: The cyclooxygenase-expressing mesenchyme resists intestinal epithelial injury by paracrine signaling
MSC-CM induces the intestinal organoid swelling by activating CFTR. A Time-lapse imaging of mouse Apoa1-mCherry; Lgr5-EGFP organoids with mouse small intestinal MSC-CM (mMSC-CM) stimulation. Scale bar, 100 μm. B Time-lapse imaging of human ileum organoids with human ileum MSC-CM (hMSC-CM) stimulation. Scale bar, 100 μm. C, D Quantification of surface area of human (C) or mouse (D) small intestinal organoids in response to the MSC-CM stimulation. The normalized area is the proportion of organoid surface area relative to the surface area at t = 0. E Time-lapse imaging and surface area quantification of CFTRinh-172 and GlyH-101-treated human ileum organoids with hMSC-CM stimulation. The human small intestinal organoids were preincubated with 50 μM CFTRinh-172 and 50 μM GlyH-101 (CFTR inhibitors) for 3 h before stimulation. Scale bar, 100 μm. F Quantification of surface area of CFTRinh-172 and GlyH-101-treated mouse small intestinal organoids with mMSC-CM stimulation. The mouse small intestinal organoids were preincubated with 50 μM CFTRinh-172 and 50 μM GlyH-101 (CFTR inhibitors) for 3 h before stimulation. G Surface area quantification of H89-treated mouse small intestinal organoids after mMSC-CM stimulation. The mouse small intestinal organoids were preincubated with 10 μM H89 (PKA inhibitor) for 6 h before stimulation. Data represent mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, unpaired two-tailed t-test (C, D), one-way ANOVA (E, F, G)