Fig. 2
From: The cyclooxygenase-expressing mesenchyme resists intestinal epithelial injury by paracrine signaling
The COX-PGE2-EP4 axis is involved in the MSC-CM-induced organoid swelling. A The PGE2 concentration of mMSC-CM, NIH3T3-CM or L cell-CM, quantified by ELISA assay. B Time-lapse imaging and surface area quantification of small intestinal organoids with mMSC-CM or PGE2 (7 nM) stimulation. Scale bar, 100 μm. C Immunofluorescent staining of EP2 and EP4 in mouse small intestine. Scale bar, 100 μm. D Quantification of surface area of E7046 or TG4-155-treated mouse small intestinal organoids with mMSC-CM stimulation. The mouse small intestinal organoids were preincubated with 10 μM E7046 (EP4 inhibitor) or 10 μM TG4-155 (EP2 inhibitor) for 72 h before stimulation. E The PGE2 concentration of SC560 or Celecoxib-treated mMSC-CM, quantified by ELISA assay. F Surface area quantification of mouse small intestinal organoids with stimulation using SC560 or Celecoxib-treated mMSC-CM. The mMSCs were preincubated with 20 nM SC560 (COX1 inhibitor), 150 nM Celecoxib (COX2 inhibitor), or both for 48 h, then the CM was collected for stimulation. Data represent mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, one-way ANOVA (B, D, E, F)