Fig. 4
From: The cyclooxygenase-expressing mesenchyme resists intestinal epithelial injury by paracrine signaling
Loss of PGE2 dampens the Muc2 expression in mouse intestinal epithelium. A Representative structures of Gli1-PtgscKO (Gli1-CreER; Ptgs1flox/flox; Ptgs2flox/flox) and Ctrl (Ptgs1flox/flox; Ptgs2flox/flox) mouse small and large intestine indicated by H&E staining. The dashed lines indicate the crypt region of small intestine. Scale bar, 100 μm. B The crypt and villus length of Gli1-PtgscKO and Ctrl mouse small intestine. C Heatmap showing differential expressed genes of small intestinal crypts between Gli1-PtgscKO and Ctrl mice, analyzed from bulk RNA-seq. D Immunofluorescent staining of Muc2 and quantification of Muc2+ cell number of Gli1-PtgscKO and Ctrl mouse small intestine. Scale bar, 100 μm. E Muc2 expressions of organoids treated with PGE2, as revealed by RT-PCR analysis. Data represent mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. not significant, unpaired two-tailed t-test (B, D), one-way ANOVA (E)